Solanum muricatum (Pepino) is a solanaceous crop gaining popularity but susceptible to biotic and abiotic stress in cultivation. Grafting has been shown to improve the plant’s adaptability to adverse conditions. The results showed that the In terms of The grafted cantaloupe eggplant showed stronger resistance to low-temperature stress, the increase in relative conductivity of cantaloupe eggplant autografted seedlings was significantly higher than that of grafted seedlings, and both MDA contents were significantly lower than that of cantaloupe eggplant autografted seedlings, and the low-temperature tolerance of the grafted seedlings became more pronounced with the prolongation of the stress time.When assessing resistance to low-temperature stress, grafted pepinos showed enhanced tolerance compared to non-grafted cuttings, likely due to synergistic effects between scion and rootstock during the grafting process. These results suggest that grafting can significantly improve pepino’s low-temperature tolerance.