Potassium is an essential macronutrient for living creatures on earth and in plants, it plays a very significant role in determining the overall health of the plants. Although potassium is present in the soil, it is present in a form that is inaccessible to the plants, and hence synthetic harmful non-eco-friendly potassium fertilizers are used. To overcome this problem, the use of eco-friendly potassium-solubilizing bacteria comes into play. The goal of the present study was to assess the potassium-solubilizing bacteria that inhabit the farm rhizosphere, which demonstrate the presence of enzymes associated with plant growth promotion and antagonistic properties. A total of thirty-four isolates were isolated from the rhizosphere. All these isolates were subjected to a potassium solubilization test on Aleksandrov agar medium, out of which fourteen were found to possess potassium solubilizing ability. On the basis of the 16S rRNA gene sequencing, the most potential potassium-solubilizing bacterium was identified as Proteus mirabilis PSCR17. The plant growth promoting abilities and production of biocontrol enzymes of this isolate were evaluated, and the results indicated, in addition to potassium solubilization, the isolate was positive for indole acetic acid production, hydrogen cyanide production, amylase, catalase, cellulase, chitinase, and protease. The use of potassium fertilizers is harmful to the environment and ecosystem; hence, this study concludes that P. mirabilis PSCR17 can be used as a substitute for chemical potassium fertilizers to improve the growth and biocontrol traits of the plants in a sustainable manner after further research.
The article’s proposed engineering uses are based on theories presented in the reviewed research articles and on findings from online investigations into companies that claim to use nanoengineering in their wares. Several pre-existing online consumer inventories and nanotechnology news were examined as part of the internet inquiry. The data about the nanoparticles (NP), or nanostructure, used in commercially available products comes from the remarks made by the manufacturer. Nanoengineered coating agents and textile additives are examples of commercial items developed for industrial clients that fall under the aforementioned uses.
Cysteine is one of the body’s essential amino acids to build proteins. For the early diagnosis of a number of diseases and biological issues, L-cysteine (L-Cys) is essential. Our study presents an electrochemical sensor that detects L-cysteine by immobilizing the horseradish peroxidase (HRP) enzyme on a reduced graphene oxide (GCE) modified glassy carbon electrode. The morphologies and chemical compositions of synthesized materials were examined using Fourier transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FESEM). The modified electrode’s electrochemical behavior was investigated using cyclic voltammetry (CV). Cyclic voltammetry demonstrated HRP/rGO/GCE has better electrocatalytic activity than bare GCE in the oxidation of L-cysteine oxidation in a solution of acetate buffer. The electrochemical sensor had a broad linear range of 0 µM to 1 mM, a 0.32 µM detection limit, and a sensitivity of 6.08 μA μM−1 cm−2. The developed sensor was successfully used for the L-cysteine detection in a real blood sample with good results.
Phytochemical and antioxidant analysis of some varieties of Capsicum was evaluated. Mature Capsicum varieties were collected across the State. The seeds were removed, sun-dried for 3 days, stored for 2 weeks at 15 ºC–25 ºC in polythene bags before planting. Saponins, tannins, flavonoids, alkaloids and cardiac glycosides were present in abundant, moderate and trace amounts. Combined anthraquinones were absent in all varieties. Yellow (0.810 ± 0.0006 µg/mL), red long dry (0.211 ± 0.0006 µg/mL) and round peppers (2.527 ± 0.0003 µg/mL) had the largest values for total phenol, flavonoids and tannins. Shombo and yellow peppers had the largest (0.270 ± 0.002 µg/mL) and least (0.102 ± 0.001 µg/mL) capsaicin content. The antioxidant activities varied across the varieties. At 100 µg/mL of methanol, yellow (45%) and round peppers (45%) had largest mean absorbances for 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Radical Scavenging Activity while sub-shombo pepper (23%) had the least. For Ferric Reducing Antioxidant Power (FRAP), yellow (0.63 ± 0.001 µg/mL) and sub-shombo peppers (0.55 ± 0.001µg/mL) had the largest and least values at 100 µg/mL of methanol. At 100 µg/mL of methanol, red long dry (0.112 ± 0.001) and shombo peppers (0.101 ± 0.001) had the largest and least values for the nitric oxide scavenging activity. This study shows that Capsicum varieties exhibit bioactive componds similarities and variations with implications in hybridization, taxonomy and conservation.
Every production day in Nigeria, and in other oil producing countries, millions of barrels of produced water is generated. Being very toxic, remediation of the produced water before discharge into environment or re-use is very essential. An eco-friendly and cost effective approach is hereby reported for remediative pre-treatment of produced water (PW) obtained from Nigerian oilfield. In this approach, Telfairia occidentalis stem extract-silver nanoparticles (TOSE-AgNPs) were synthesized, characterized and applied as bio-based adsorbent for treating the PW in situ. The nanoparticles were of average size 42.8 nm ± 5.3 nm, spherical to round shaped and mainly composed of nitrogen and oxygen as major atoms on the surface. Owing to the effect of addition of TOSE-AgNPs, the initially high levels (mg/L) of Total Dissolved Solids (TDS), Biological Oxygen Demand (BOD) and TSS of 607, 3.78 and 48.4 in the PW were reduced to 381, 1.22 and 19.6, respectively, whereas DO and COD improved from 161 and 48.4 to 276 and 19.6 respectively, most of which fell within WHO and US-EPA safe limits. Particularly, the added TOSE-AgNPs efficiently removed Pb (II) ions from the PW at temperatures between 25 ℃ to 50 ℃. Removal of TOSE-AgNPs occurred through the adsorption mechanism and was dependent contact time, temperature and dose of TOSE-AgNPs added. Optimal remediation was achieved with 0.5 g/L TOSE-AgNPs at 30 ℃ after 5 h contact time. Adsorption of Pb (Ⅱ) ions on TOSE-AgNPs was spontaneous and physical in nature with remediation efficiency of over 82% of the Pb (Ⅱ) ions in solution. Instead of discarding the stem of Telfairia occidentalis, it can be extracted and prepared into a new material and applied in the oilfield as reported here for the first time.
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