Fusarium oxysporum is one of the most common soil-dwelling pathogens that causes wilt on tomato crops. There is a plethora of literature on Fusarium, but comparatively fewer studies exist when it comes down to Fusarium and tomato together. Different experimental models on tomato infestation with Fusarium were tried. Three nitrogen compounds: KNO₃, (NH₄)₂ SO₄ and Urea, and three media: Malt Extract Agar, Potato Dextrose Agar and V8 were used in this study. Concentrations of each of the three nitrogen compounds were varied by preparing solutions of 0.5 g/L, 1.0 g/L and 1.5 g/L of each compound. Radial growth is measured in mm while mass is determined in mg. V8 medium was found to be the best medium for the growth as compared to tested media. The best nitrogen source for the growth of the isolate was potassium nitrate. The optimum temperature for the growth of the fungus was found to be in the range of 25–30.