This review comprehensively summarizes various preparatory methods of polymeric bone scaffolds using conventional and modern advanced methods. Compilations of the various fabrication techniques, specific composition, and the corresponding properties obtained under clearly identified conditions are presented in the commercial formulations of bone scaffolds in current orthopedic use. The gaps and unresolved questions in the existing database, efforts that should be made to address these issues, and research directions are also covered. Polymers are unique synthetic materials primarily used for bone and scaffold applications. Bone scaffolds based on acrylic polymers have been widely used in orthopedic surgery for years. Polymethyl methacrylate (PMMA) is especially known for its widespread applications in bone repair and dental fields. In addition, the PMMA polymers are suitable for carrying antibiotics and for their sustainable release at the site of infection.

Recent technological advances in the fields of biomaterials and tissue engineering have spurred interest in biopolymers for various biomedical applications. The advantage of biopolymers is their favorable characteristics for these applications, among which proteins are of particular importance. Proteins are explored widely for 3D bioprinting and tissue engineering applications, wound healing, drug delivery systems, implants, etc., and the proteins mainly available include collagen, gelatin, albumin, zein, etc. Zein is a plant protein abundantly present in corn endosperm, and it is about 80% of total corn protein. It is a highly renewable source, and zein has been reported to be applicable in different industrial applications. Lately, it has gained attention in biomedical applications. This research interest in zein is on account of its biocompatibility, non-toxicity, and certain unique physico-chemical properties. Zein comes under the GRAS category and is considered safe for biomedical applications. The hydrophobic nature of this protein gives it an added advantage and has wider applications in drug delivery. This review focuses on details about zein protein, its properties, and potential applications in biomedical sectors.

Three-dimensional (3D) bioprinting is a promising technological approach for various applications in the biomedical field. Natural polymers, which comprise the majority of 3D printable “bioinks”, have played a crucial role in various 3D bioprinting technologies during the layered 3D manufacturing processes in the last decade. However, the polymers must be customized for printing and effector function needs in cancer, dental care, oral medicine and biosensors, cardiovascular disease, and muscle restoration. This review provides an overview of 3D bio-printed natural polymers—commonly employed in various medical fields—and their recent development.