With its inherent characteristics of decentralization, immutability, and transparency, blockchain technology presents a promising opportunity to revolutionize the South African food supply chains. Blockchain technology, with its decentralized, immutable, and secure nature, offers solutions to these challenges by improving traceability and accountability across the supply chain. This study investigates the role of blockchain technology in enhancing transparency in the food supply chain among small and medium enterprises in South Africa. SMEs form a critical part of the country’s agri-food sector but face challenges such as food fraud, inefficient inventory management, and lack of transparency, which impact food safety and trust. The research adopts a mixed-method approach, utilizing the Technology-Organization-Environment framework and Institutional Theory to explain blockchain adoption among SMEs. The results demonstrate that blockchain-enabled practices, such as smart contracts, records traceability, production tracking, and distribution monitoring, significantly enhance supply chain transparency. The findings highlight blockchain’s potential to increase operational efficiency, regulatory compliance, and stakeholder trust. This research provides valuable insights for policymakers and practitioners, emphasizing the need for regulatory support and strategic investment in blockchain solutions to promote sustainability and competitiveness in the agri-food sector.