Objective: This study investigates the efficacy and safety of epidural infiltration with drugs and an oxygen-ozone mixture for treating cervicobrachialgia due to disc-radicular conflict or on a degenerative basis, utilizing both retrospective analysis and direct visualization techniques. Methods: A retrospective study involving 10 patients treated with epidural infiltrations of an oxygen-ozone mixture and cortisone was conducted. The procedures were performed under CT guidance to ensure precise delivery and to monitor the diffusion of the injected substances. Pain levels were assessed using the Numerical Rating Scale (NRS) and treatment efficacy was evaluated based on symptom relief and reduction in NSAID intake. Results: Significant pain reduction was observed post-treatment, with median NRS scores decreasing from 9 (baseline) to 2 (follow-up), and a significant decrease in on-demand NSAID intake. Only one minor complication of a headache was reported. The study also demonstrated the ability of ozone to diffuse through the epidural adipose tissue, potentially enhancing treatment efficacy. Conclusion: The combined use of an oxygen-ozone mixture and cortisone for epidural infiltration is an effective and safe treatment for cervicobrachialgia, offering significant pain relief and minimizing the risk associated with traditional epidural injections. This technique presents a viable non-surgical option for patients suffering from disc-radicular conflict or degenerative conditions.

Objective: To study the changes of growth, physiological and absorption characteristics of Pinus bungeana under ozone (O₃) stress, to elucidate the correlations among the indicators, and to determine its degree of response to O₃. Methods: The growth, physiological characteristics and O₃ uptake capacity of Pinus bungeana seedlings were measured in an open-top O₃ fumigation manual control experiment with three concentration gradients (NF: normal atmospheric O₃ concentration, NF40: normal atmospheric O₃ concentration plus 40 nmlol/mol; NF80: normal atmospheric O₃ concentration plus 80 nmol/mol), and the relationships between the characteristics of Pinus bungeana under different O₃ concentrations were investigated with correlation analysis, redundancy analysis and analysis of variance. Results: (1) Plant height growth (ΔH), diameter growth at 50 cm (ΔDBH), stomatal size (S), stomatal density (M), stomatal opening (K), stomatal conductance (G_s), net photosynthetic rate (P_n), transpiration rate (E_t), water use efficiency (WUE), maximum photochemical efficiency (F_v/F_m), chlorophyll content (CHL), whole tree water consumption (W), and O₃ uptake rate () all decreased with the increase of O₃ concentration; while intercellular CO₂ concentration () and relative conductivity (L) increased with the increase of O₃ concentration; (2) growth indicators of Pinus bungeana under O₃ stress (ΔH, ΔDBH) were the most correlated with O₃ uptake status (, W), followed by photosynthetic indicators (, WUE, ,, ) and growth indicators (ΔH, ΔDBH) and stomatal characteristics (K, M, S) under O₃ stress, some physiological indicators (L, ) were relatively weakly correlated with photosynthesis (, WUE,,, ) and stomatal (K, M, S); (3) all the indicators of Pinus bungeana were significantly different under O₃ treatments of NF and NF80 (P < 0.05), ΔH, ΔDBH, M, CHL, , , W and were most significantly different under NF and NF40 treatments, and K, S, WUE, , , , L were more significantly different under NF40 and NF80 treatments. Conclusion: The experiment proved that the growth of Pinus bungeana was slowed, photosynthetic capacity was reduced, and the absorption capacity of O₃ was further reduced by long-term exposure to high concentration of O₃. The growth of Pinus bungeana was most correlated with the changes of O₃ absorption characteristics, and the stomatal characteristics were most correlated with photosynthetic physiological characteristics, and the reduction of photosynthetic capacity etc. further led to the curtailment of its growth.