This study aims to explore the connotation of Daoist medicine culture and investigate its relationship with modern medicine. Exploring the connotation of Daoist medicine culture is beneficial for advocating a healthy lifestyle, improving people’s physical and mental health, promoting individual comprehensive development, and enhancing happiness. By drawing wisdom and experience from Daoist medicine, inheriting various medical methods such as herbal treatment, acupuncture, massage, and integrating the concept of integrated Chinese and Western medicine into modern medicine, not only can treatment effectiveness be improved, but also interdisciplinary communication and cooperation can be promoted, thus driving the innovation and development of medical knowledge.