In wealthy nations, biofuel usage has grown in importance as a means of addressing climate change concerns, ensuring energy security, and promoting agricultural development. Because they understand the potential advantages of biofuel for rural development and job creation, governments have created policies and legislation to encourage the production of biofuel. However, the province of Limpopo hasn’t fully taken advantage of the potential to use biofuel production as a vehicle for job development, despite a higher demand for the fuel. There is currently a lack of understanding of the role of biofuel in promoting local development in developing regions. For this reason, this study made use of semi-structured interviews to explore how biofuel production can be used as an instrument for Local Economic Development (LED) in the Limpopo province of South Africa. The research investigated the determinants of empowerment that could impact the commercial feasibility of biofuel production in the province. It also identified the need for human resource development to get workers ready for jobs in Limpopo’s biofuel sector. The results showed that, provided certain conditions were met, the production of biofuel in Limpopo may be a useful instrument for creating local jobs. By highlighting the potential for job creation and the importance of human resource development, this research aims to facilitate evidence-based decision-making that can harness biofuel production for sustainable rural development in the region. The value of this study lies in its contribution to the understanding of biofuel’s role in LED, offering actionable insights for policymakers and stakeholders in Limpopo.