The xanthorrhiza species of the genus Arracacia belongs to the Apiaceae family and is known for its ability to generate tuberous reservoir roots that are harvested annually and marketed fresh in South American countries such as Colombia, Brazil, Venezuela, Peru, Bolivia and Ecuador. In Colombia, arracacha is planted mainly in 15 departments and the regional cultivars are differentiated by the color of the leaves, petiole and tuberous root, the best known being amarilla común or paliverde, yema de huevo, and cartagenera. There are studies that have characterized regional materials by applying a limited number of descriptors, but they do not allow knowing the morphology and phenotypic differentiation of each one; therefore, their definition and characterization constitute a support in breeding programs that allow the efficient use of the genetic potential and increase the knowledge about the diversity of cultivars. Phenotypic characterization and description of three cultivars was performed during two production cycles (2016 and 2018) in two phases (vegetative and productive) applying 74 morphological variables (42 qualitative and 32 quantitative) organized in seven groups of variables: plant, leaf, leaflet, petiole, propagule, stock and tuberous root. A factorial analysis for mixed data (FAMD) was performed, which incorporated a multivariate analysis with all variables and identified 11 discriminant variables, 8 qualitative and 3 quantitative, which can be used in processes of characterization of arracacha materials. A morphological description of each cultivar was made, which means that this is the first complete characterization study of regional arracacha materials in Colombia.