This paper aims to explore the impact of V-Girls APP on the improvement of female college students' Health literacy and its mechanism. Using a questionnaire survey method, the survey subjects were female students from a certain university. The results showed that using the V-Girls app can significantly improve the health knowledge level, health behavior habits, and mental health status of female college students. Further analysis reveals that the impact mechanisms of V-Girls APP mainly include cognitive mechanisms, social support mechanisms, and behavioral guidance mechanisms. The results of this study provide new ways and ideas for improving female college students' Health literacy.