A longstanding interest in bone tissue engineering is the development of new bio-scaffolds that can be manufactured on a large scale with high throughput at low cost. Here, we report a low-cost and systematically optimized hydrothermal synthesis for producing Mo-doped potassium titanate nanofibers with high structural purity. This new nanosynthesis is based on bone tissue growth on an undoped titanate nanowires-entangled scaffold, as previously reported by our team. The morphological and structural characterization data suggest that the crystal structure of Mo-doped titanate nanofibers closely resembles that of the undoped ones. This resemblance is potentially valuable for assessing the role of Mo dopants in engineering bone tissue.