The internationalization of higher education began to take shape during the period of the Republic of China. This trend manifested in various forms and encompassed a rich array of activities, including the construction of teaching staffs, the exchange of international students, and the presence of overseas scholars giving lectures in China. Between 1899 and 1945, Japanese institutions sent nearly 200 academic overseas students to China. With the establishment and improvement of the internal system of universities in the Republic of China, these students were able to study and interact with Chinese scholars. The forms of communication were diverse, the content was rich, and the channels were smooth, making the process lively and interesting with distinct characteristics of the era. Consequently, this group became both participants and witnesses in the internationalization process of universities in the Republic of China. However, the full-scale Anti-Japanese War disrupted the internationalization of universities, causing it to deviate from its normal trajectory. Some Japanese academic overseas students who had previously studied in China became instruments of Japanese imperialism’s cultural invasion and educational colonization. These students played a significant role in promoting the “alternative internationalization” of universities in the Republic of China. In short, examining the involvement of Japanese academic overseas students providing us a unique insight into the general situation and processes of internationalization at universities in the Republic of China during different historical periods.