According to the World Health Organization (WHO), breast cancer is among the most common cancers worldwide. Most of the anticancer agents have been showing a variety of side effects. Recently, bacterial proteins have been investigated as promising anticancer agents. Azurin is a bacterial cupredoxin protein secreted from Pseudomonas aeruginosa and has been reported as a potent multi-targeting anticancer agent, which makes it an appropriate candidate for drug delivery. Azurin may be delivered to cancer cells using different carriers like polymeric micro and nanoparticles. In the present study, azurin was extracted from the bacterial host and loaded into chitosan particles. Then its effect on MCF-7 cell line was investigated. Chitosan-azurin particles were made using the ion gelation method. Results showed that chitosan-azurin particles are about 200 nm, and the loading of the protein in particles did not affect its integrity. The MTT assay showed a significant reduction in cell viability in azurin and chitosan-azurin-treated cells. The toxicity level after 5 days was 63.78% and 82.53% for free azurin and chitosan-azurin-treated cells, respectively. It seems using an appropriate carrier system for anticancer proteins like azurin is a promising tool for developing low side effect anticancer agents.

This study analyzes the dynamic relationships between tourism, gross domestic product (GDP) per capita, exports, imports, and carbon dioxide (CO₂) emissions in five South Asian countries. A VAR-based Granger causality test is performed with time series data from Bangladesh, India, Nepal, Pakistan, and Sri Lanka. According to the results, both bidirectional and unidirectional relationships among tourism, economic growth, and carbon emissions are investigated. Specifically, tourism significantly impacts GDP per capita in Pakistan, Sri Lanka, and Nepal, yet it has no effect in Bangladesh or India. However, the GDP per capita shows a unidirectional relationship with tourism in Bangladesh and India. The unidirectional causal relationship from exports and imports to tourism in the context of India and a bidirectional relationship in the case of Nepal. In Pakistan, it is observed that exports have a one-way influence on tourism. The result of the panel Granger test shows a significant causal association between tourism, economic growth, and trade (import and export) in five South Asian economies. Particularly, there is a bidirectional causal relationship between GDP per capita and tourism, and a significant unidirectional causal relationship from CO₂ emissions, exports, and imports to tourism is explored. The findings of this study are helpful for tourism stakeholders and policymakers in the region to formulate more sustainable and effective tourism strategies.

During and after any disaster, a situation report (SITREP) is prepared, based on the Daily Incident Updates (DIU), as an initial decision support information base. It is observed that the decision support system and best practices are not optimized through the available formal reporting on disaster incidents. The rapidly evolving situation, misunderstood terms, inaccurate data and delivery delays of DIU are challenges to the daily SITREP. Multiple stakeholders stipulated with different tasks should be properly understood for the SITREP to initiate relevant response tasks. To fill this research gap, this paper identifies the weaknesses of the current practice and discusses the upgrading of the incident-reporting process using a freely available software tool, enabling further visualization, and producing a comprehensive timely output to share among the stakeholders. In this case, “Power-BI” (a data visualization software) is used as a 360-degree view of useful metrics—in a single place, with real-time updates while being available on all devices for operational decision-making. When a dataset is transformed into several analytical reports and dashboards, it can be easily shared with the target users and action groups. This article analyzed two sources of data, namely the Disaster Management Center (DMC) and the National Disaster Relief Service Center (NDRSC) of Sri Lanka. Senior managers of disaster emergencies were interviewed and explored social media to develop a scheme of best practices for disaster reporting, starting from just before the occurrence, and following the unfolding sequence of the disasters. Using a variety of remotely acquired imageries, rapid mapping, grading, and delineating impacts of natural disasters, were made available to concerned users.

Definitive diagnosis of Craniosynostosis (CS) with computed tomography (CT) is readily available, however, exposure to ionizing radiation is often a hard stop for parents and practitioners. Lowering head CT radiation exposure helps mitigate risks and improves diagnostic utilization. The purpose of the study is to quantify radiation exposure from head CT in patients with CS using a ‘new’ (ultra-low dose) protocol; compare prior standard CT protocol; summarize published reports on cumulative radiation doses from pediatric head CT scans utilizing other low-dose protocols. A retrospective study was conducted on patients undergoing surgical correction of CS, aged less than 2 years, between August 2014 and February 2022. Cumulative effective dose (CED) in mSv was calculated, descriptive statistics were performed, and mean ± SD was reported. A literature search was conducted describing cumulative radiation exposure from head CT in pediatric patients and analyzed for ionizing radiation measurements. Forty-four patients met inclusion criteria: 17 females and 27 males. Patients who obtained head CT using the ‘New’ protocol resulted in lower CED exposure of 0.32 mSv ± 0.07 compared to the prior standard protocol at 5.25 mSv ± 2.79 (p < 0.0001). Five studies specifically investigated the reduction of ionizing radiation from CT scans in patients with CS via the utilization of low-dose CT protocols. These studies displayed overall CED values ranging from 0.015 mSv to 0.77 mSv. Our new CT protocol resulted in 94% reduction of ionizing radiation. Ultra-low dose CT protocols provide similar diagnostic data without loss of bone differentiation in CS and can be easily incorporated into the workflow of a children’s hospital.

The present study assessed the potential of sediment loading in Beteni, Lauruk, Andheri, and Harpan sub-watersheds of Phewa Lake and estimated the sediment yield in the year 2020. Morphometry, land use/land cover, geology, climate, and human and development factors of the sub-watersheds were studied to assess the potential of sediment loading in the sub-watersheds. SRTM DEM was used for the computation of morphometric parameters and land use/land cover maps were prepared by using Landsat imagery. Geology, rainfall data, census data, and road maps were collected from various secondary sources. The sediment yields of the four sub-watersheds in the year 2020 were estimated by measuring the sediment volume deposited in the sediment retention ponds at the outlet of each sub-watershed. Results indicated that Beteni had the highest potential for sediment loading, while Harpan had the lowest. Likewise, the sediment yields for Beteni, Lauruk, Andheri, and Harpan sub-watersheds in 2020 were estimated at 1,420.67 m³/km²/year, 2,280.14 m³/km²/year, 1,666.77 m³/km²/year, and 766.42 m³/km²/year, respectively. To reduce sedimentation in Phewa Lake, it is recommended to regularly maintain siltation dams and construct check dams along the drainage slopes, alongside other soil conservation measures and appropriate land use practices in the upstream areas of the sub-watersheds.

In marginalized ecosystem-dependent rural communities, access to ecosystem services plays a crucial role in achieving sustainable livelihoods. This study was conducted to find out the influence of various livelihood capital components on the access mechanism for forest-based Provisioning Services (PS) in some selected villages of the Gosaba Block on the fringes of the Sundarban. The contribution of the livelihood capitals to gain access to Provisioning Services (PS) was identified using factor analysis on 160 households, selected through cluster random sampling. The sustainability levels of livelihood capitals were analyzed using the Prescott-Allen method (2001). The natural, financial, social, and physical capitals were significantly below average, while the human capital was close to average. Enhancement of human, physical, financial, and social capital, ease in issuing Biometric Fisherman cards for entering forests, flexibility in borrowing loans, and ecotourism by involving local villagers must be encouraged to enhance forest-based provisioning services in the near future.