Introduction: Growth, yield and quality of okra (Abelmoschus esculentus (L.) Moench) are related to fertilizer application, being nitrogen (N) the most outstanding, due to its direct relationship with photosynthesis and vegetative growth of the plant. Objective: The objective was to evaluate the agronomic and productivity characteristics of okra as a function of N dose. Materials and methods: The study was conducted at the experimental area of Campus Gurupi, the Universidad Federal de Tocantins (UFT), Brazil, in two planting periods (autumn/winter and spring/summer). The experimental design used was randomized block design (RBD) with six treatments (50, 100, 150, 150, 200 and 250 kg N ha^-1) and four replications. Urea was used as a source of N. The characteristics evaluated were: productivity, average fruit mass, height and plant chlorophyll index. Results: Productivity and plant height were superior in the fall/winter crop. Mean fruit mass and chlorophyll index were not influenced by planting time. For productivity, a linear response was obtained with increasing dose up to the limit of the N dose used (250 kg ha^-1), with a mean value higher than 14 t of fruit. Mean mass and plant height responded linearly to increasing N dose. Nitrogen affected the chlorophyll index, with maximum values of 45.96 and 47.19, observed in the two evaluation periods. Conclusion: Planting time and N content in the soil interacted with plant height, being favorable in the period without precipitation. N influenced all the characteristics, demonstrating the importance of nitrogen fertilization in the development of okra plants.

The micro staring hyperspectral imager can simultaneously acquire two spatial and one spectral images, and only record the external orientation elements of the entire hyperspectral image rather than the external orientation elements of each frame of the image, which avoids the geometric instability during scanning, effectively solves the problem of large geometric deformation of the small line scanning hyperspectral imager, and is suitable for the small UAV load platform with unstable attitude. At present, most of the research focuses on the radio-metric correction method of line scan hyperspectral imager. The application time of staring hyperspectral imager is short, and there is no mature data processing re-search at home and abroad, which hinders the application of UAV micro staring hyperspectral imaging system. In this paper, the calibration method of the linearity and variability of the radiation response of the micro staring hyperspectral imager on the UAV is studied, and the effectiveness of this method is quantitatively evaluated. The results show that the hyperspectral image has obvious vignetting effect and strip phenomenon before the correction of radiation response variability. After the correction, the radiation response variation coefficient of pixels in different bands decreases significantly, and the vignetting effect and image strip decrease significantly. In this paper, a multi-target radiometric calibration method is proposed, and the accuracy of radiometric calibration is verified by comparing the calibrated hyperspectral image spectrum with the measured ground object spectrum of the ground spectrometer. The results show that the calibration results of the multi-target radiometric calibration method show better results, especially for the near-infrared band, and the difference with the surface reflectance measured by the spectrometer is small.

The wide distribution of the common beech (Fagus sylvatica) in Europe reveals its great adaptation to diverse conditions of temperature and humidity. This interesting aspect explains the context of the main objective of this work: to carry out a dendroclimatic analysis of the species Fagus sylvatica in the Polaciones valley (Cantabria), an area of transition with environmental conditions from a characteristic Atlantic type to more Mediterranean, at the southern limit of its growth. The methodology developed is based on the analysis of 25 local chronologies of growth rings sampled at different altitudes along the valley, generating a reference chronology for the study area. Subsequently, the patterns of growth and response to climatic variations are estimated through the response and correlation function, and the most significant monthly variables in the annual growth of the species are obtained. Finally, these are introduced into a Geographic Information System (GIS) where they are cartographically modeled in the altitudinal gradient through multivariate analysis, taking into account the different geographic and topographic variables that influence the zonal variability of the species response. The results of the analyses and cartographic models show which variables are most determinant in the annual growth of the species and the distribution of its climatic response according to the variables considered.

Objective: To study the changes of growth, physiological and absorption characteristics of Pinus bungeana under ozone (O₃) stress, to elucidate the correlations among the indicators, and to determine its degree of response to O₃. Methods: The growth, physiological characteristics and O₃ uptake capacity of Pinus bungeana seedlings were measured in an open-top O₃ fumigation manual control experiment with three concentration gradients (NF: normal atmospheric O₃ concentration, NF40: normal atmospheric O₃ concentration plus 40 nmlol/mol; NF80: normal atmospheric O₃ concentration plus 80 nmol/mol), and the relationships between the characteristics of Pinus bungeana under different O₃ concentrations were investigated with correlation analysis, redundancy analysis and analysis of variance. Results: (1) Plant height growth (ΔH), diameter growth at 50 cm (ΔDBH), stomatal size (S), stomatal density (M), stomatal opening (K), stomatal conductance (G_s), net photosynthetic rate (P_n), transpiration rate (E_t), water use efficiency (WUE), maximum photochemical efficiency (F_v/F_m), chlorophyll content (CHL), whole tree water consumption (W), and O₃ uptake rate () all decreased with the increase of O₃ concentration; while intercellular CO₂ concentration () and relative conductivity (L) increased with the increase of O₃ concentration; (2) growth indicators of Pinus bungeana under O₃ stress (ΔH, ΔDBH) were the most correlated with O₃ uptake status (, W), followed by photosynthetic indicators (, WUE, ,, ) and growth indicators (ΔH, ΔDBH) and stomatal characteristics (K, M, S) under O₃ stress, some physiological indicators (L, ) were relatively weakly correlated with photosynthesis (, WUE,,, ) and stomatal (K, M, S); (3) all the indicators of Pinus bungeana were significantly different under O₃ treatments of NF and NF80 (P < 0.05), ΔH, ΔDBH, M, CHL, , , W and were most significantly different under NF and NF40 treatments, and K, S, WUE, , , , L were more significantly different under NF40 and NF80 treatments. Conclusion: The experiment proved that the growth of Pinus bungeana was slowed, photosynthetic capacity was reduced, and the absorption capacity of O₃ was further reduced by long-term exposure to high concentration of O₃. The growth of Pinus bungeana was most correlated with the changes of O₃ absorption characteristics, and the stomatal characteristics were most correlated with photosynthetic physiological characteristics, and the reduction of photosynthetic capacity etc. further led to the curtailment of its growth.

The food industry progressively requires innovative and environmentally safe packaging materials with increased physical, mechanical, and barrier properties. Due to its unique properties, cellulose has several potential applications in the food industry as a packaging material, stabilizing agent, and functional food ingredient. A coffee pod is a filter of cellulosic, non-rigid, ready-made material containing ground portions and pressed coffee prepared in dedicated machines. In our study, we obtained, with homogenization and sonication, cellulose micro/nanoparticles from three different coffee pods. It is known that nanoparticulate systems can enter live cells and, if ingested, could exert alterations in gastrointestinal tract cells. Our work aims to investigate the response of HT-29 cells to cellulose nanoparticles from coffee pods. In particular, the subcellular effects between coffee-embedded nanocellulose (CENC) and cellulose nanoparticles (NC) were compared. Finally, we analysed the pathologic condition (Cytolethal Distending Toxin (CDT) from Campylobacter jejuni) on the same cells conditioned by NC and CENC. We evidenced that, for the cellular functional features analysed, NC and CENC pre-treatments do not worsen cell response to the C. jejuni CDT, also pointing out an improvement of the autophagic flux, particularly for CENC preconditioning.

The possibility of preoperative prediction of pathologic complete response in rectal cancer has been studied in order to identify patients who would respond to neoadjuvant therapy and to individualize therapeutic strategies. Endoscopic ultrasound of the rectum is an accurate method for the evaluation of local tumor and lymph node invasion. Objective: To evaluate the potential of endoscopic ultrasound as a predictor of complete pathological response to neoadjuvant treatment in patients with locally advanced rectal cancer. Material and methods: Retrospective study of patients with rectal cancer from January 2014 to December 2016. Results: We obtained a statistical association between T stage by endoscopic ultrasound and complete pathological response (p = 0.015). It is not so for N, sphincter involvement, circumferential involvement and maximum tumor thickness (p = 0.723, p = 0.510, p = 0.233 and p = 0.114, respectively). When multivariate logistic regression analysis was applied to assess the degree of influence of the predictor variables on pathologic response, none of these variables was associated with complete pathologic response. Conclusion: Prediction of pathologic complete response in rectal cancer has been considered as the crucial point upon which treatments for rectal cancer could be individualized. So far, no imaging method has been able to demonstrate efficacy in predicting complete pathologic response, and in turn there is no direct association between any endosonographic finding that can accurately predict it.