Objective: To determine the presence of bacteria by means of microbiological analysis on the surfaces contacted by the operator during the taking and processing of intraoral radiographs at different times of the day in the Oral Radiology Service of the UPCH. Materials and methods: Nine surfaces of the oral radiology service were sampled. The samples were taken at two times by the same investigator; at the beginning and the end of the activities in the service, the surfaces were swabbed with Trypticase Soy Broth (TSB). The samples were inoculated and incubated in three culture media (Plate Count Agar, Lamb’s Blood Agar and Cetrimide Agar). Then the respective Colony Forming Unit (CFU) count was performed and Gram staining was also performed. Results: A high concentration of bacteria (4180 CFU/mL) and fungi was found in the oral radiology service. Gram-positive cocci were the most frequently found microorganisms and gram-negative bacilli were less frequently found. Conclusions: There is a high contamination of bacteria in the oral radiology service. When the activities are completed, the number of bacteria decreases, but the variety of bacteria increases.

In order to promote the application of noise map in high-speed railway noise management, the high-speed railway noise map drawing technology based on the combination of noise prediction model and geographic information system (GIS) is studied. Firstly, according to the distribution characteristics of noise sources and line structure characteristics of high-speed railway, the prediction model of multi equivalent sound sources and the calculation method of sound barrier insertion loss of high-speed railway are optimized; secondly, a three-dimensional geographic information model of a high-speed railway is built in GIS software, and the railway noise prediction technology based on the model is developed again; then, the noise of discrete nodes is calculated, and the continuous noise distribution map is drawn by spatial interpolation. The research results show that the comparison error between the noise map of a high-speed railway drawn by this technology and the measured results is less than 1 dB (A), which verifies the accuracy and practicality of the high-speed railway noise map, and can be used as a reference for the railway noise management department to formulate noise control countermeasures.

The national park with Chinese characteristics is the highest level of protection of a kind of natural protection, its establishment marks the park will implement the strictest ecological protection means. It is of great value to construct the utilization system of national park resources under the new natural protected area system in the new era to avoid the misunderstanding of “ecological protection only” and explore how to carry out the sustainable utilization of resources in the reform of national park system and mechanism. According to the analytic hierarchy process (AHP) and Delphi method, the evaluation framework, indicators, reference standards and weights of resource utilization under the national park system were determined in combination with the requirements of constructing the protected natural area system and the total value of resource ecosystem services (including harvest value, existence value and future value). Based on the application research of Bawangling zone of Hainan Tropical Rainforest National Park, the optimal resource utilization system in the future was proposed, and two optimization strategies of ecological adjustment of resource utilization system and construction of suitable resource utilization system were put forward.

The structure and diversity of tree species in a temperate forest in northwestern Mexico was characterized. Nine sampling sites of 50 × 50 m (2,500 m²) were established, and a census of all tree species was carried out. Each individual was measured for total height and diameter at breast height. The importance value index (IVI) was obtained, calculated from the variable abundance, dominance and frequency. The diversity and richness indices were also calculated. A total of 12 species, four genera and four families were recorded. The forest has a density of 575.11 individuals and a basal area of 23.54/m². The species of Pinus cooperi had the highest IVI (79.05%), and the Shannon index of 1.74.

The smallest administrative unit of the sixth national census-township (town) is selected as the basic unit, the population spatial distribution characteristics at the township (town) level in karst mountainous areas of northwest Guangxi are analyzed by using Lorenz curve and spatial correlation analysis method, and the influence intensity of natural factors on regional population spatial distribution is detected by using geographic detector method. The results show that: 1. the spatial distribution of population at the township (town) level has the characteristics of imbalance, showing generally significant positive correlation and certain aggregation; 2. There are significant differences in the impact of the spatial distribution of various natural factors on the population distribution. For the towns without karst distribution in the northwest and central south of the study area, the population density increases with the increase of factors conducive to human residence, but the average population density is only 79 people/km². In the towns with karst distribution in the East and south, the spatial distribution of population density and natural factors is not a simple increase or decrease relationship, but fluctuates with the change of karst distribution area. 3. The factor detection results of the geographic detector show that the altitude has the greatest impact on the spatial distribution of population. The interactive detection results show that the impact intensity of any two natural factors after superposition and interaction presents nonlinear enhancement and two factor enhancement. It can be seen that the karst mountain area in northwest Guangxi is similar to other areas. Altitude is one of the main factors affecting the spatial distribution of population, but the river network density and unique geological landform of karst mountain area have a strong catalytic effect on the spatial distribution of population. The superposition and interaction with other factors can further strengthen the impact on population distribution.

The CO₂  heat pump air conditioning system of new energy vehicle is designed, and the vehicle model of CO₂  heat pump module and heat management system is established based on KULI simulation. The effects of refrigerant charge, running time and compressor speed on the heat pump air conditioning system is studied, and the energy consumption is compared with the PTC heating system and the CO₂ heat pump air conditioning system without waste heat recovery. The results show that the optimal charge for full-service operation is 750 g; increasing the compressor speed can increase the cooling capacity, so that the refrigerant temperature in the passenger compartment and battery inlet can quickly reach the appropriate temperature, but the COP_h, COP_c are reduced by 2.5% and 1.8% respectively. By comparing it with PTC heating and CO₂ heat pump air conditioning systems without waste heat recovery, it is found that the energy consumption of this system is only for the PTC heating systems 42.5%, without waste heat recovery carbon dioxide heat pump air conditioning system of 86.6%. It greatly saves energy, but also increased the waste heat recovery function, so that the system supply air temperature increased by 26%, improve passenger cabin comfort. This  provides a reference for the future experimental research of CO₂ heat pump air conditioning and heat management system.