Digital technology and the e-commerce industry are deeply integrated, and the pace of digital transformation of various industries is accelerated; in this context, it has given rise to the rise of e-commerce operated by rural residents, and as a digital depression, digital liter_x005facy has become one of the most important influences on rural e-commerce practitioners’ future survival and development. Through literature survey, expert survey method and other methods, the indicators of their digital literacy evaluation were designed. And the questionnaire on digital literacy level is prepared according to the derived indicators, and the survey data of 436 rural e-commerce practitioners in Yiwu are statistically analyzed; finally, the path of enhancement is given.