According to the World Health Organization (WHO), breast cancer is among the most common cancers worldwide. Most of the anticancer agents have been showing a variety of side effects. Recently, bacterial proteins have been investigated as promising anticancer agents. Azurin is a bacterial cupredoxin protein secreted from Pseudomonas aeruginosa and has been reported as a potent multi-targeting anticancer agent, which makes it an appropriate candidate for drug delivery. Azurin may be delivered to cancer cells using different carriers like polymeric micro and nanoparticles. In the present study, azurin was extracted from the bacterial host and loaded into chitosan particles. Then its effect on MCF-7 cell line was investigated. Chitosan-azurin particles were made using the ion gelation method. Results showed that chitosan-azurin particles are about 200 nm, and the loading of the protein in particles did not affect its integrity. The MTT assay showed a significant reduction in cell viability in azurin and chitosan-azurin-treated cells. The toxicity level after 5 days was 63.78% and 82.53% for free azurin and chitosan-azurin-treated cells, respectively. It seems using an appropriate carrier system for anticancer proteins like azurin is a promising tool for developing low side effect anticancer agents.

This article explores ethnomedicine or traditional medication written in palm-leaf manuscripts in Lombok, West Nusa Tenggara. Most of the manuscripts in Lombok are written on palm leaf or lontar. One genre of palm-leaf manuscripts is USADA or traditional medication. These USADA manuscripts, serving as guides for traditional healers (dukun and balian), contain valuable information on diseases, treatments, herbal remedies, and incantations. The study reveals that ethnomedicine remains a relevant and respected practice in Lombok, often considered on par with modern medicine. Many residents rely on traditional healers for healthcare, particularly for minor illnesses. The research also highlights the living nature of the manuscript tradition, with continued recitation, copying, and teaching of these texts to younger generations. However, challenges persist in preserving these manuscripts and promoting wider appreciation for this unique cultural heritage.