Cysteine is one of the body’s essential amino acids to build proteins. For the early diagnosis of a number of diseases and biological issues, L-cysteine (L-Cys) is essential. Our study presents an electrochemical sensor that detects L-cysteine by immobilizing the horseradish peroxidase (HRP) enzyme on a reduced graphene oxide (GCE) modified glassy carbon electrode. The morphologies and chemical compositions of synthesized materials were examined using Fourier transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FESEM). The modified electrode’s electrochemical behavior was investigated using cyclic voltammetry (CV). Cyclic voltammetry demonstrated HRP/rGO/GCE has better electrocatalytic activity than bare GCE in the oxidation of L-cysteine oxidation in a solution of acetate buffer. The electrochemical sensor had a broad linear range of 0 µM to 1 mM, a 0.32 µM detection limit, and a sensitivity of 6.08 μA μM−1 cm−2. The developed sensor was successfully used for the L-cysteine detection in a real blood sample with good results.
Currently, there is a significant gap between the training objectives and the actual situation of electromechanical talents in higher vocational colleges. Many teachers in electromechanical departments do not meet the required qualifications and are unable to adapt to the developments of the new era. The talent training mode is insufficiently comprehensive, and the criteria for talent assessment are not unified. In response to these issues, it is necessary to promptly change the mindset, innovate educational ideas, focus on the present while planning for the future, clarify training objectives, adopt a dual education model that integrates production and education, strengthen the faculty, utilize their potential, and improve the overall educational quality to provide guarantees for talent development.
The chemical reinforcement of sandy soils is usually carried out to improve their properties and meet specific engineering requirements. Nevertheless, conventional reinforcement agents are often expensive; the process is energy-intensive and causes serious environmental issues. Therefore, developing a cost-effective, room-temperature-based method that uses recyclable chemicals is necessary. In the current study, poly (styrene-co-methyl methacrylate) (PS-PMMA) is used as a stabilizer to reinforce sandy soil. The copolymer-reinforced sand samples were prepared using the one-step bulk polymerization method at room temperature. The mechanical strength of the copolymer-reinforced sand samples depends on the ratio of the PS-PMMA copolymer to the sand. The higher the copolymer-to-sand ratio, the higher the sample’s compressive strength. The sand (70 wt.%)-PS-PMMA (30 wt.%) sample exhibited the highest compressive strength of 1900 psi. The copolymer matrix enwraps the sand particles to form a stable structure with high compressive strengths.
In the current context of new engineering, the teaching of the course "Civil Engineering Construction Organization and Management" should be targeted and focused. In terms of setting up the course content, schools need to engage in extensive communication and cooperation with enterprises and industry associations, and integrate more practical education elements into the teaching methods to ensure that students can achieve a unity of knowledge and action; In relevant course teaching, teachers should also introduce more ideological and political elements to improve students' ideological and moral literacy. This article analyzes and explores the teaching reform of the course "Civil Engineering Construction Organization and Management" in the context of the new engineering discipline.
Before the formal construction of a building, it is necessary to conduct an effective survey of the engineering geology, hydrogeology, and other contents within the construction area. The survey work is not only an important part of the early stage of engineering construction for engineering management personnel, but also an important factor in ensuring safety and stability during the construction process. Therefore, in order to effectively avoid various geological risks during the construction process or after the completion of the building, the preliminary engineering geological survey work is of great significance. In the process of engineering geological exploration, hydrogeological issues are also important exploration projects. This article will explore and study hydrogeological issues, analyze the water physical properties of rock and soil, and the impact of hydrogeological issues on the project, and then propose effective measures to do well in engineering geological exploration.
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