Instant and accurate evaluation of drug resistance in tumors before and during chemotherapy is important for patients with advanced colon cancer and is beneficial for prolonging their progression-free survival time. Here, the possible biomarkers that reflect the drug resistance of colon cancer were investigated using proton magnetic resonance spectroscopy (1H-MRS) in vivo. SW480[5-fluorouracil(5-FU)-responsive] and SW480/5-FU (5-FU-resistant) xenograft models were generated and subjected to in vivo 1H-MRS examinations when the maximum tumor diameter reached 1–1.5 cm. The areas under the peaks for metabolites, including choline (Cho), lactate (Lac), glutamine/glutamate (Glx), and myoinositol (Ins)/creatine (Cr) in the tumors, were analyzed between two groups. The resistancerelated protein expression, cell morphology, necrosis, apoptosis, and cell survival of these tumor specimens were assessed. The content for tCho, Lac, Glx, and Ins/Cr in the tumors of the SW480 group was significantly lower than that of the SW480/5-FU group (p < 0.05). While there was no significant difference in the degree of necrosis and apoptosis rate of tumor cells between the two groups (p > 0.05), the tumor cells of the SW480/5-FU showed a higher cell density and larger nuclei. The expression levels of resistance-related proteins (P-gp, MPR1, PKC) in the SW480 group were lower than those in the SW480/5-FU group (p < 0.01). The survival rate of 5-FU-resistant colon cancer cells was significantly higher than that of 5-FUresponsive ones at 5-FU concentrations greater than 2.5 μg/mL (p < 0.05). These results suggest that alterations in tCho, Lac, Glx1, Glx2, and Ins/Cr detected by 1H-MRS may be used for monitoring tumor resistance to 5-FU in vivo.

Given the heavy workload faced by teachers, automatic speaking scoring systems provide essential support. This study aims to consolidate technological configurations of automatic scoring systems for spontaneous L2 English, drawing from literature published between 2014 and 2024. The focus will be on the architecture of the automatic speech recognition model and the scoring model, as well as on features used to evaluate phonological competence, linguistic proficiency, and task completion. By synthesizing these elements, the study seeks to identify potential research areas, as well as provide a foundation for future research and practical applications in software engineering.

Forests have ecological functions in water conservation, climate regulation, environmental purification, soil and water conservation, biodiversity protection and so on. Carrying out forest ecological quality assessment is of great significance to understand the global carbon cycle, energy cycle and climate change. Based on the introduction of the concept and research methods of forest ecological quality, this paper analyzes and summarizes the evaluation of forest ecological quality from three comprehensive indicators: forest biomass, forest productivity and forest structure. This paper focuses on the construction of evaluation index system, the acquisition of evaluation data and the estimation of key ecological parameters, discusses the main problems existing in the current forest ecological quality evaluation, and looks forward to its development prospects, including the unified standardization of evaluation indexes, high-quality data, the impact of forest living environment, the acquisition of forest level from multi-source remote sensing data, the application of vertical structural parameters and the interaction between forest ecological quality and ecological function.

A method for studying the resilience of energy and socio-ecological systems is considered; it integrates approaches developed at the International Institute of Applied Systems Analysis and the Melentyev Institute of Energy Systems (MESI) of the Siberian Branch of the Russian Academy of Sciences. The article discusses in detail the methods of using intelligent information technologies, in particular semantic technologies and knowledge engineering (cognitive probabilistic modeling), which the authors propose to use in assessing the risks of natural and man-made threats to the resilience of the energy sector and social and ecological systems. More attention is paid to the study and adaptation of the integral indicator of quality of life, which makes it possible to combine these interdisciplinary studies.

A systemic and synthetic review of the anatomy of the temporomandibular joint in magnetic resonance imaging was developed for its evaluation. The temporomandibular joint is an anatomical structure composed of bones, muscles, ligaments and an articular disc that allows important physiological movements, such as mandibular opening, closing, protrusion, retrusion and lateralization. Magnetic resonance imaging is an imaging technique that does not use ionizing radiation and is more specific for the evaluation and interpretation of soft tissues, due to its high resolution, so it has an important role in the diagnosis of various maxillofacial pathologies, which is why the dentist should have knowledge of the structures and functions of the temporomandibular joint through magnetic resonance imaging. The review demonstrates the importance of magnetic resonance imaging in the study of the anatomy of the temporomandibular joint, in addition to mentioning the advantages provided by this imaging technique such as its good detail of the soft tissues in its different sequences and the non-use of ionizing radiation to obtain its images.

The UN agenda of Sustainable Development Goals (SDGs) 2015–2030 is a holistic approach. Universities play an important role in dissemination of quality knowledge, developing the skills and attitudes of a large number of youth across the world. Though the emphasis on Education for Sustainable Development (ESD) started as early as 1992, yet Universities adopted the concept of Green Campus integrating the environmental, social and economic aspects of sustainability quite recently. In developing countries including Pakistan, the Green Campus Initiatives (GCI) have not been implemented in the majority of the Universities. Northern Pakistan comprising Azad Jammu & Kashmir (AJ&K) and Gilgit Baltistan (GB) faces multiple challenges including Climate Impacts at the top. The fragile ecosystem of the region requires more sustainable initiatives at the University and community levels. In this research, the readiness of the seven universities located in Northern Pakistan have been assessed for GCI on the basis scanning of the websites and questionnaire survey of the relevant stakeholders. The results have shown that there is little commitment of resources for sustainability from senior management, lack of awareness in faculty & staff and less research focus on the related themes of green campus. The co-curricular activities in universities are not linked with sustainability and there are no incentives for faculty, staff and students to this end. It has been recommended that Green Campus Framework may be developed for Pakistani Mountain Universities, with commitment from leaders of the universities and allocation of sufficient resources for development of sustainable campuses. The Higher Education Commission of Pakistan (HEC) needs to allocate special funds for promoting GCI across universities in Pakistan.