Cysteine is one of the body’s essential amino acids to build proteins. For the early diagnosis of a number of diseases and biological issues, L-cysteine (L-Cys) is essential. Our study presents an electrochemical sensor that detects L-cysteine by immobilizing the horseradish peroxidase (HRP) enzyme on a reduced graphene oxide (GCE) modified glassy carbon electrode. The morphologies and chemical compositions of synthesized materials were examined using Fourier transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FESEM). The modified electrode’s electrochemical behavior was investigated using cyclic voltammetry (CV). Cyclic voltammetry demonstrated HRP/rGO/GCE has better electrocatalytic activity than bare GCE in the oxidation of L-cysteine oxidation in a solution of acetate buffer. The electrochemical sensor had a broad linear range of 0 µM to 1 mM, a 0.32 µM detection limit, and a sensitivity of 6.08 μA μM−1 cm−2. The developed sensor was successfully used for the L-cysteine detection in a real blood sample with good results.
Preserving roads involves regularly evaluating government policy through advanced assessments using vehicles with specialized capabilities and high-resolution scanning technology. However, the cost is often not affordable due to a limited budget. Road surface surveys are highly expected to use low-cost tools and methods capable of being carried out comprehensively. This research aims to create a road damage detection application system by identifying and qualifying precisely the type of damage that occurs using a single CNN to detect objects in real time. Especially for the type of pothole, further analysis is to measure the volume or dimensions of the hole with a LiDAR smartphone. The study area is 38 province’s representative area in Indonesia. This research resulted in the iRodd (intelligent-road damage detection) for detection and classification per type of road damage in real-time object detection. Especially for the type of pothole damage, further analysis is carried out to obtain a damage volume calculation model and 3D visualization. The resulting iRodd model contributes in terms of completion (analyzing the parameters needed to be related to the road damage detection process), accuracy (precision), reliability (the level of reliability has high precision and is still within the limits of cost-effective), correct prediction (four-fifths of all positive objects that should be identified), efficient (object detection models strike a good balance between being able to recognize objects with high precision and being able to capture most objects that would otherwise be detected-high sensitivity), meanwhile, in the calculation of pothole volume, where the precision level is established according to the volume error value, comparing the derived data to the reference data with an average error of 5.35% with an RMSE value of 6.47 mm. The advanced iRodd model with LiDAR smartphone devices can present visualization and precision in efficiently calculating the volume of asphalt damage (potholes).
The use of artificial intelligence (AI) in the detection and diagnosis of plant diseases has gained significant interest in modern agriculture. The appeal of AI arises from its ability to rapidly and precisely analyze extensive and complex information, allowing farmers and agricultural experts to quickly identify plant diseases. The use of artificial intelligence (AI) in the detection and diagnosis of plant diseases has gained significant attention in the world of agriculture and agronomy. By harnessing the power of AI to identify and diagnose plant diseases, it is expected that farmers and agricultural experts will have improved capabilities to tackle the challenges posed by these diseases. This will lead to increased effectiveness and efficiency, ultimately resulting in higher agricultural productivity and reduced losses caused by plant diseases. The use of artificial intelligence (AI) in the detection and diagnosis of plant diseases has resulted in significant benefits in the field of agriculture. By using AI technology, farmers and agricultural professionals can quickly and accurately identify illnesses affecting their crops. This allows for the prompt adoption of appropriate preventative and corrective actions, therefore reducing losses caused by plant diseases.
To achieve sustainable development, detailed planning, control and management of land cover changes that occur naturally or by human caused artificial factors, are essential. Urban managers and planners need a tool that represents them the information accurate, fast and in exact time. In this study, land use changes of 3 periods, 1994-2002, 2002-2009, 2009-2015 and predictions of 2009, 2015 and 2023 were assessed. In this paper, Maximum Likelihood method was used to classify the images, so that after evaluation of accuracy, amount of overall accuracy for images of 2013 was 85.55% and its Kappa coefficient was 80.03%. To predict land use changes, Markov-CA model was used after assessing the accuracy, and the amount of overall accuracy for 2009 was 82.57% and for 2015 was 93.865%. Then web GIS application was designed via map server application and evoked shape files through map file and open layers to browser environment and for design of appearance of website CSS, HTML and JavaScript languages were used. HTML is responsible for creating the foundation and overall structure of webpage but beautifying and layout design on CSS.
Retinal disorders, such as diabetic retinopathy, glaucoma, macular edema, and vein occlusions, are significant contributors to global vision impairment. These conditions frequently remain symptomless until patients suffer severe vision deterioration, underscoring the critical importance of early diagnosis. Fundus images serve as a valuable resource for identifying the initial indicators of these ailments, particularly by examining various characteristics of retinal blood vessels, such as their length, width, tortuosity, and branching patterns. Traditionally, healthcare practitioners often rely on manual retinal vessel segmentation, a process that is both time-consuming and intricate, demanding specialized expertise. However, this approach poses a notable challenge since its precision and consistency heavily rely on the availability of highly skilled professionals. To surmount these challenges, there is an urgent demand for an automatic and efficient method for retinal vessel segmentation and classification employing computer vision techniques, which form the foundation of biomedical imaging. Numerous researchers have put forth techniques for blood vessel segmentation, broadly categorized into machine learning, filtering-based, and model-based methods. Machine learning methods categorize pixels as either vessels or non-vessels, employing classifiers trained on hand-annotated images. Subsequently, these techniques extract features using 7D feature vectors and apply neural network classification. Additional post-processing steps are used to bridge gaps and eliminate isolated pixels. On the other hand, filtering-based approaches employ morphological operators within morphological image processing, capitalizing on predefined shapes to filter out objects from the background. However, this technique often treats larger blood vessels as cohesive structures. Model-based methods leverage vessel models to identify retinal blood vessels, but they are sensitive to parameter selection, necessitating careful choices to simultaneously detect thin and large vessels effectively. Our proposed research endeavors to conduct a thorough and empirical evaluation of the effectiveness of automated segmentation and classification techniques for identifying eye-related diseases, particularly diabetic retinopathy and glaucoma. This evaluation will involve various retinal image datasets, including DRIVE, REVIEW, STARE, HRF, and DRION. The methodologies under consideration encompass machine learning, filtering-based, and model-based approaches, with performance assessment based on a range of metrics, including true positive rate (TPR), true negative rate (TNR), positive predictive value (PPV), negative predictive value (NPV), false discovery rate (FDR), Matthews's correlation coefficient (MCC), and accuracy (ACC). The primary objective of this research is to scrutinize, assess, and compare the design and performance of different segmentation and classification techniques, encompassing both supervised and unsupervised learning methods. To attain this objective, we will refine existing techniques and develop new ones, ensuring a more streamlined and computationally efficient approach.
Proper understanding of LULC changes is considered an indispensable element for modeling. It is also central for planning and management activities as well as understanding the earth as a system. This study examined LULC changes in the region of the proposed Pwalugu hydropower project using remote sensing (RS) and geographic information systems (GIS) techniques. Data from the United States Geological Survey's Landsat satellite, specifically the Landsat Thematic Mapper (TM), the Enhanced Thematic Mapper (ETM), and the Operational Land Imager (OLI), were used. The Landsat 5 thematic mapper (TM) sensor data was processed for the year 1990; the Landsat 7 SLC data was processed for the year 2000; and the 2020 data was collected from Operation Land Image (OLI). Landsat images were extracted based on the years 1990, 2000, and 2020, which were used to develop three land cover maps. The region of the proposed Pwalugu hydropower project was divided into the following five primary LULC classes: settlements and barren lands; croplands; water bodies; grassland; and other areas. Within the three periods (1990–2000, 2000–2020, and 1990–2020), grassland has increased from 9%, 20%, and 40%, respectively. On the other hand, the change in the remaining four (4) classes varied. The findings suggest that population growth, changes in climate, and deforestation during this thirty-year period have been responsible for the variations in the LULC classes. The variations in the LULC changes could have a significant influence on the hydrological processes in the form of evapotranspiration, interception, and infiltration. This study will therefore assist in establishing patterns and will enable Ghana's resource managers to forecast realistic change scenarios that would be helpful for the management of the proposed Pwalugu hydropower project.
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