Cucumis sativus is an important vegetable crop in the world. Agrobacterium mediated transgenic technology is an important means to study plant gene function and variety improvement. In order to further accelerate the transgenic research and breeding process of cucumber, aiming at the Agrobacterium mediated genetic transformation method of cucumber, this paper expounds the research progress and existing problems of Agrobacterium mediated transgenic cucumber from the aspects of influencing factors of cucumber regeneration ability, genetic transformation conditions and various added substances in the process, and prospects the future of improving the efficiency of cucumber genetic transformation and the application of safety screening markers, in order to provide reference for cucumber stress resistance breeding and fruit quality improvement.

The expansion of road networks, taken place during the last decades, was driven by technological progress and economic growth. The most innovative products of this trend—modern motorways and international road corridors—provide an excellent level of service, traffic safety and necessary information to travelers. However, despite this undeniable progress, major impediments and respective challenges to road authorities and operators still remain. The present paper analytically presents the main current challenges in the road engineering field, namely: a) financing new projects, b) alternative energy resources, especially renewable energy, c) serviceability, including maintenance of road infrastructure, traffic congestion and quality of the network, d) climate change hazards due to greenhouse gas emissions increase, e) environmental impacts, f) safety on roads, streets and motorways, and g) economy and cost-effectiveness. In each country and over each network, challenges and concerns may vary, but, in most cases, competent authorities, engaged in road development policies, have to deal with most of these issues. The optimization of the means to achieve the best results seems to be an enduring stake. In the present paper, the origin and the main features of these challenges are outlined as well as their tendency to get amplified or diminished under the actual evolving economic conditions worldwide, where growth alternates with crisis and social hardship. Moreover, responses, meant to provide solutions to the said challenges, are suggested, including research findings of Aristotle University and innovative technological achievements, to drive the transition to a more sustainable future.

Knowledge of the presence of heavy metals in soils of agricultural areas is important to prevent their accumulation in cultivated plants. The objective of the present investigation was to evaluate the total concentrations and fractions of heavy metals Cd, Pb, Zn, Fe, Mn, Ni, Cu, Cr and Co in the tobacco-growing area of Pinar del Río, Cuba and their relationship with the physicochemical properties of soil. For the study, 59 samples of three types of soils were collected at 20 cm depth. The pseudo-total concentrations of metals in the soils are low and lower than the prevention values registered for Cuban soils. In general, the heavy metals studied present a high affinity for the most stable fractions of the soil, which means a low risk of transfer to the tobacco crop or accumulation in groundwater. The pseudo-total concentrations of heavy metals were low, below the alert values established for soils in the region. The heavy metals studied were mainly associated with the residual fraction, the second fraction with the highest association with metals was that linked to manganese and iron oxides. The principal component analysis showed that their main source is pedogenetic and that these elements are closely related to cation exchange capacity and calcium content.

Through the combination of the geographic information systems (GIS) and the integrated information model, the stability of regional bank slope was comprehensively evaluated. First, a regional bank slope stability evaluation index system was established through studying seven selected factors (slope grade, slope direction, mountain shadow, elevation, stratigraphic lithology, geological structure and river action) that have an impact on the stability of the slope. Then, each factor was rasterized by GIS. According to the integrated information model, the evaluation index distribution map based on rasterized factors was obtained to evaluate the stability of the regional bank slope. Through the analysis of an actual project, it was concluded that the geological structure and stratigraphic lithology have a significant impact on the evaluation results. Most of the research areas were in the relatively low stable areas. The low and the relatively low stable areas accounted for 15.2% and 51.5% of the total study area respectively. The accuracy of slope evaluation results in the study area reached 95.41%.

Sustainable ocean tourism is required to establish a balance between the environmental, economic, social and cultural aspects of ocean tourism development. Sustainable ocean tourism also contributes to local and national economies, enhancing the quality of social life and protecting the ecology. Sustainable ocean tourism expands the positive contribution of tourism to biodiversity conservation and poverty reduction and aims to attain the common goals of sustainable developments for ocean tourism. Sustainable ocean tourism is possible due to the roles of regulators and private and government institutions. Government policies, regulations and guidelines play vital roles towards achieving the sustainability of ocean tourism. However, the role of institutions also cannot be ignored, which provide support in the innovation of technologies and the implementation of policies. The paper targets to investigate the roles of regulations, policies and institutions in the sustainability of ocean tourism. A primary online survey on the perception of tourism experts was conducted for this study using Google Forms. The tourism experts were invited from all over the world to participate in the survey. The study received a total of 33 responses, out of which only 30 valid responses were considered. Using the Tobit regression model, the study found that, while regulations in India relative to foreign countries significantly boost the sustainability of ocean tourism, government policies and public institutions in India relative to foreign countries remain insignificant in predicting the sustainability of ocean tourism. Therefore, government policies and public institutions in India need to be revised and reformulated to make them important drivers of the sustainability of ocean tourism.