Modified chitosan hybrids were obtained via chemical reaction of chitosan with two pyrazole aldehyde derivatives to produce two chitosan Schiff bases, Cs-SB1, and Cs-SB2, respectively. FTIR spectroscopy and scanning electron microscopy confirmed both chemical structures and morphology of these Schiff bases. Thermal gravimetric analysis showed an improvement of thermal properties of these Schiff bases. Both chitosan Schiff bases were evaluated in a batch adsorption approach for their ability to remove Cu(II) ions from aqueous solutions. Energy dispersive X-ray for the Schiff bases adsorbed metal ions in various aqueous solutions was performed to confirm the existence of adsorbed metal ions on the surface substrate and their adsorptive efficiency for Cu(II) ions. Results of the batch adsorption method showed that prepared Schiff bases have good ability to remove Cu(II) ions from aqueous solutions. The Langmuir isotherm equation showed a better fit for both adsorbents with regression coefficients (R² = 0.97 and 0.99, respectively) with maximum adsorption capacity for Cu(II) of 10.33 and 39.84 mg/g for Cs-SB1 and Cs-SB2, respectively. All prepared compounds, pyrazoles and two chitosan Schiff bases, showed good antimicrobial activity against three Gram +ve bacteria, three Gram –ve bacteria and Candida albicans, with varying degrees when compared to the standard antimicrobial agents.

Artificial intelligence chatbots can be used to conduct research effectively and efficiently in the fifth industrial revolution. Artificial intelligence chatbots are software applications that utilize artificial intelligence technologies to assist researchers in various aspects of the research process. These chatbots are specifically designed to understand researchers’ inquiries, provide relevant information, and perform tasks related to data collection, analysis, literature review, collaboration, and more. The purpose of this study is to investigate the use of artificial intelligence chatbots for conducting research in the fifth industrial revolution. This qualitative study adopts content analysis as its research methodology, which is grounded in literature review incorporating insights from the researchers’ experiences with utilizing artificial intelligence. The findings reveal that researchers can use artificial intelligence chatbots to produce quality research. Researchers are exposed to various types of artificial intelligence chatbots that can be used to conduct research. Examples are information chatbots, question and answer chatbots, survey chatbots, conversational agents, peer review chatbots, personalised learning chatbots and language translation chatbots. Artificial intelligence chatbots can be used to perform functions such as literature review, data collection, writing assistance and peer review assistance. However, artificial intelligence chatbots can be biased, lack data privacy and security, limited in creativity and critical thinking. Researchers must be transparent and take in consideration issues of informed content and data privacy and security when using artificial intelligence chatbots. The study recommends a framework on artificial intelligence chatbots researchers can use to conduct research in the fifth industrial revolution.