A total of 25 SSR primers were screened on 37 putative F₁s derived from the five different crosses. Identified cross specific highly informative SSRs primers, i.e., 14 for the first cross, 10 for the second, 12 for the third and 6 each for fourth and fifth crosses. For the first cross Bhagwa × Daru 17, four primers (HvSSRT_375, NRCP_SSR9, NRCP_SSR12 and NRCP_SSR92) were found to be highly informative with higher 100% hybrid purity index (HPI), PIC (~0.52), and observed heterozygosity (Ho, range 0.87–0.93) values, and two F₁s namely H₁ and H₂ were found to be highly heterotic with a heterozygosity index (HI) of 92.85%. Similarly, for Bhagwa × Nana, three primers (HvSSRT_375, HvSSRT_605 and NRCP_SSR19) had higher HPI (70%–100%), PIC (0.52–0.69), and Ho (0.75–0.33) values, and three F₁s H₁, H₂, and H₄ had 70% (HI). For Bhagwa × IC318712, four SSRs (HvSSRT_254, HvSSRT_348, HvSSRT_826 and NRCP_SSR95) had higher Ho (~0.83), HPI (100%) and PIC (~0.52) values, and four F₁s H₂, H₇, H₉, and H₁₀ showed 91.66% (HI). For Bhagwa × Nayana, HvSSRT_605, HvSSRT_826, and HvSSRT_432, and for Ganesh × Nayana, HVSSRT_375, HVSSRT_605, and HvSSRT_826 were found informative. These markers will be highly useful in developing maps of populations.

The expansion of road networks, taken place during the last decades, was driven by technological progress and economic growth. The most innovative products of this trend—modern motorways and international road corridors—provide an excellent level of service, traffic safety and necessary information to travelers. However, despite this undeniable progress, major impediments and respective challenges to road authorities and operators still remain. The present paper analytically presents the main current challenges in the road engineering field, namely: a) financing new projects, b) alternative energy resources, especially renewable energy, c) serviceability, including maintenance of road infrastructure, traffic congestion and quality of the network, d) climate change hazards due to greenhouse gas emissions increase, e) environmental impacts, f) safety on roads, streets and motorways, and g) economy and cost-effectiveness. In each country and over each network, challenges and concerns may vary, but, in most cases, competent authorities, engaged in road development policies, have to deal with most of these issues. The optimization of the means to achieve the best results seems to be an enduring stake. In the present paper, the origin and the main features of these challenges are outlined as well as their tendency to get amplified or diminished under the actual evolving economic conditions worldwide, where growth alternates with crisis and social hardship. Moreover, responses, meant to provide solutions to the said challenges, are suggested, including research findings of Aristotle University and innovative technological achievements, to drive the transition to a more sustainable future.

The characteristics of agricultural products are influenced by the ecosystem, from the perspective of biotic and abiotic factors, which produce in the plant physiological responses and in turn in the fruit unique physicochemical properties, which are the basis for designations of origin and strategies to add value to the product in the current market. In the present work, ten cocoa materials (Theobroma cacao L.) were selected for their outstanding productivity (FSV41, FLE3, FEAR5, FSA12, FEC2, SCC23, SCC80, SCC55, ICS95 and CCN51), which were established in the departments of Santander (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.) and Huila (931 m a.s.l.). These were established in the departments of Santander (931 m a.s.l.), Huila (885 m a.s.l.) and Arauca (204 m a.s.l.), the main cocoa-producing areas in Colombia. For the evaluation of the physical characteristics of the collected materials, 21 quantitative descriptors were used to determine the physical variability of the fruit according to clone and place of collection. The data collected were analyzed by means of Pearson’s correlation matrix and principal component analysis, it was possible to identify those descriptors that contribute most to the variability among materials (ear index, diameter length ratio, seed weight and diameter, and fruit weight and length). In addition, it was possible to verify the effect of the place of harvest on the physical characteristics of the materials, high-lighting the importance of the adaptation study prior to the planting of the cocoa material, with the objective of guaranteeing a premium, productive and quality cocoa crop for the industry, which is competitive in the market.

Depression is a mental disorder caused by various causes with significant and persistent depressed mood as the main clinical feature, and is the most common mental illness worldwide and in our country. The number of patients with depression worldwide was as high as 350 million in 2017, and the number of patients with depression in our country was nearly 100 million in 2019. The greatest danger of depression is self-injurious and suicidal behaviour, and this behaviour carries a high medical burden. Medication is the most costly treatment for depression in China, and while it is an effective way to treat patients with depression, it has many side effects and poor patient compliance. Non-pharmacological treatments commonly used in clinical practice include physiotherapy and psychotherapy. Physiotherapy is commonly used in non-convulsive electroconvulsive therapy, but its clinical efficacy is uncertain and it can also cause adverse effects such as heart failure and arrhythmias, which are poorly tolerated by patients. Psychotherapy is also a common non-pharmacological therapy. Cognitive therapy is a common form of psychotherapy, but the cycle of cognitive therapy is too long, the cost to the patient is high, and the patient’s cognitive ability has certain requirements. Music therapy is a combination of art and science. It is a cross-discipline that combines body, movement, dance and psychology and is a method of psychotherapy that has biological, psychological and social functions to compensate for deficiencies. Music therapy sees a fundamental connection between mind and body and emphasises that what affects the body also affects the mind. When mind-body integration is lacking, individuals will suffer from a variety of psychological disorders. Therefore, the core principles of music therapy emphasise that holistic individual health is embodied in the integration of mind and body, that body movement is expressive and communicative, and that music therapy uses body movement as a method of assessing the individual and as a means of clinical intervention.

In this study, ‘Xinli No. 3’, ‘Shengli rootstock’, ‘Shenli rootstock’ and ‘Shengzhen No. 1’ were used as rootstock, and ‘Jinchun No. 39’ cucumber was used as scion to study the effects of different rootstock on the yield and quality of grafted cucumber, and to select high quality rootstock suitable for cucumber grafting. Different rootstock affected the survival rate, phenology, the height of plant, stem diameter, growth potential, yield and quality of cucumber grafting. Among them, the survival rate of ‘Shenli rootstock’ grafted cucumber is the highest, and the growth of ‘Shengzhen No. 1’ grafted cucumber is relatively the strongest. There was no significant difference in fruit tuber, melon edge, thorn color and pulp crispness between self-rooted seedling (CK) and each rootstock grafting combination. The average yield of ‘Xinli No. 3’ grafted cucumber plot was not significantly different from that of self-rooted seedlings (CK). The length of ‘Shenli rootstock’ and ‘Shengli rootstock’ grafted cucumber was significantly higher than that of self-rooted seedlings (CK), and the length of ‘Shengzhen No. 1’ Grafted Cucumber was significantly higher than that of self-rooted seedlings (CK). The contents of vitamin C and soluble protein of ‘Shengli rootstock’, ‘Shenli rootstock’ and ‘Shengzhen No. 1’ grafted cucumber were significantly higher than those of self-rooted seedlings (CK), and the contents of soluble sugar were lower than those of self-rooted seedlings (CK). Therefore, ‘Shengzhen No. 1’ and ‘Jinchun No. 39’ have strong compatibility with cucumber. As rootstocks, the grafted cucumber plants not only have strong growth potential and high yield, but also significantly increase the content of soluble protein and vitamin C.