Cysteine is one of the body’s essential amino acids to build proteins. For the early diagnosis of a number of diseases and biological issues, L-cysteine (L-Cys) is essential. Our study presents an electrochemical sensor that detects L-cysteine by immobilizing the horseradish peroxidase (HRP) enzyme on a reduced graphene oxide (GCE) modified glassy carbon electrode. The morphologies and chemical compositions of synthesized materials were examined using Fourier transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FESEM). The modified electrode’s electrochemical behavior was investigated using cyclic voltammetry (CV). Cyclic voltammetry demonstrated HRP/rGO/GCE has better electrocatalytic activity than bare GCE in the oxidation of L-cysteine oxidation in a solution of acetate buffer. The electrochemical sensor had a broad linear range of 0 µM to 1 mM, a 0.32 µM detection limit, and a sensitivity of 6.08 μA μM⁻¹ cm⁻². The developed sensor was successfully used for the L-cysteine detection in a real blood sample with good results.

Currently, important efforts are being made to improve governability and governance by combining the monopoly of state decisions with the collaboration of diverse actors in public practice. Based on the above, the purpose of this article is to analyze the evolution of conceptual approaches to both terms over the last 23 years, examining scientific production by author authors, journals, and countries. The methodology was based on a bibliometric analysis: First, the WoS and Scopus databases were searched. Subsequently, scientometric techniques and the Science Tree methodology were used to identify patterns, structures, and trends, to understand the progress and behavior of scientific production, and to measure the quantity and quality of research that has addressed these issues from different perspectives. This study examined governability and governance publications and their annual citations to assess their impact and analyzed the total output of both datasets to identify similarities and differences in governability and governance research. The findings reveal that the number of publications and citations in this field is increasing, with the United States being the most academically influential country and the journal Marine Policy being the most prominent in ranking. These data provide key information for decision-makers, researchers, and academics for future debate and discussion toward operationalizing the concepts at the practical level of action, management, and the functioning of government structures.

The experiments were carried out to validate an analytical method and to examine the impact of various decontaminating solutions on the removal of acephate residues from okra. Acephate analysis was performed using HPLC-UV, and sample extraction was done using the QuEChERS method. Method validation encompassed assessing specificity, linearity, precision, accuracy, as well as limits of detection (LOD) and quantification (LOQ). The method exhibited excellent linearity with R² values ≥ 0.99. LOD and LOQ were determined at 0.5 µg mL⁻¹ and 2 µg mL⁻¹, respectively. The results indicated average recoveries ranging from 80.2% to 83.3% with a % RSD below 5%. The decontamination procedures include rinsing with running tap water, soaking in lukewarm water, 2% CH₃COOH, 1% NaCl, 5% NaHCO₃, 0.01% KMnO₄, and in commercially available decontamination products such as nimwash, veggie clean, and arka herbiwash for a duration 10 minutes. Among all the treatments, soaking in nimwash solution showed remarkable effectiveness (96.75% removal), followed by veggie clean (94.97% removal) and arka herbiwash (95.80% removal). Washing okra samples in running tap water was found to be the least effective compared to other treatments.

The ultimate objective of the study was to investigate the effects of being landlocked on the living standards in Sub-Saharan African (SSA) countries from 1991 to 2019. Adopting the two-step estimation technique of System GMM (generalized method of moments), the study found that being landlocked has a negative and significant effect on the living standards in SSA countries when using GDP per capita as the living standard measure. Moreover, the historical living standard experiences of SSA countries have a positive and significant influence on the current living standard level. In addition, the population growth rate has a positive and significant effect on the living standards in SSA countries. On the other hand, the official exchange rate, broad money as a percentage of GDP, and inflation have a negative and significant effect on the living standards in SSA countries. Generally, the estimated result reveals the existence of a significant variation in the living standards in landlocked and coastal SSA countries. This study suggests that regional integration between landlocked and transit countries should be improved to minimize entry costs and increase access to global markets for landlocked countries. We argue that this study is of interest to landlocked and coastal countries to increase trade integration and promote the development of both groups, and it will contribute to the scarce empirical evidence.

Heavy metal contaminated soil due to industrial, agricultural and municipal activities is becoming a global concern. Heavy metals severely affect plants, animals and human health. A suitable technology is necessary for heavy metals removal because it cannot self-decomposition as organic compounds. Among the various technologies surveyed, phytoremediation is one of the safest, most innovative, environmental friendly and cost-effective approach for heavy metals removal. Nevertheless, traditional phytoremediation practices pose some limitations such as long processing time, unstable treatment efficiency and limited application at large scale. In many methods proposed to improve phytoremediation, integrated phytoremediation has been studied in the recent years. Integrated phytoremediation use chelating agents and phytohormones to enhance phytoremediation. This is an environmentally safe, saving time and relative high effective method. Results showed that the association of a metal ion and a chelating agent to form chelates helps to maintain the availability of metals in the soil for the uptake of plants. Phytohormones supply nutrients for the soil to support vegetable growth. Therefore, integrated phytoremediation is a promising solution to overcome the disadvantages of conventional phytoremediation. It should be taken commercialization and need more applied projects in this field to demonstrate and clarify the real potential of this technology. In view of above, this manuscript reviews the mechanism and the efficiency of integrated phytoremediation for heavy metals in contaminated soil to give an overview of this technology.

Medicinal herbs have been extensively utilized in the remediation of various health conditions. Dialium guineense fruit pulp, also well known as Velvet Tamarind is widely consumed in West Africa for its dietary and medicinal properties. The study aims to analyze the phytochemical constituents, vitamin content and the in vitro antioxidant effect of Dialium guineense fruit pulp (DGFP). The phytochemical constituents, vitamins (C, E, B1-12) composition, and in vitro antioxidant activity were examined utilizing standardized analytical methods. The qualitative and quantitative phytochemical screening of the fruit pulp of Dialium guineense was also carried out; the result indicated the presence of flavonoids, alkaloids, saponins, tannins, terpenoids, phenols, steroids, and cardiac glycosides in varying concentrations. The vitamin composition revealed that vitamin C was higher than other vitamins in the fruit pulp. The DPPH (2,2-diphenyl-1-picrylhydrazyl) and nitric oxide scavenging assay showed high radical scavenging activity while the FRAP (Ferric reducing antioxidant power) assay revealed significant reducing power. This indicates that Dialium guineense fruit pulp has potential health benefits.