Leaf litter decomposition and carbon release patterns in five homegarden tree species of Kumaun Himalaya viz. Ficus palmata, Ficus auriculata, Ficus hispida, Grewia optiva and Celtis austalaris were investigated. The study was carried out for 210 days by using litter bag technique. In the current investigation, the duration needed for desertion of the original biomass of diverse leaf litter varied from 150 to 210 days and specifies a varying pattern of decomposition and carbon release among the species. Grewia optiva took the longest time to decompose (210 days) while Ficus hispida decomposed more quickly than rest of the species (150 days). The relative decomposition rate (RDR) was reported highest in Ficus hispida (0.009-0.02 g-1d-1) and lowest in Grewia optiva (0.008-0.004 g-1d-1). Carbon (%) in remaining litter was in the order: Ficus auriculata (24.4 %) >Ficus hispida (24.3%) > Celtis austaralis (19.8%) > Ficus palmata (19.7%) > Grewia optiva (19%). The relationship between percentage weight loss and time elapsed showed the significant negative correlation with carbon release pattern in all the species. Releasing nutrients into the soil through the decomposition of homegarden tree residuals is a crucial ecological function that also regulates the nutrient recycling in homegarden agroforestry practices.

The H3N2 influenza virus is spiking dramatically, which is a major concern worldwide and in India. The multifunctional hetero-trimer influenza virus RNA-dependent RNA polymerase (RdRP) is involved in the generation of viral mRNA and is crucial for viral infectivity, which is directly related to the virus’s ability to survive. The goal of the current work was to use molecular docking to determine how the RdRP protein might be affected by powerful bioactive chemicals found in Calotropis gigantia latex. By applying CB-dock 2 analysis and 2D interactions, an in-silico docking study was conducted using a GC-FID (gas chromatography with flame-ionization detection) based composition profile. Tocospiro A (15%), Amyrin (7%), and Gombasterol A were found by GC-FID to be the main phytocompounds in the latex of Calotropis gigantia. The docking result showed that ligands were effectively bound to RdRP. According to interaction studies, RdRP/ligand complexes create hydrogen bonds, van der Waals forces, pi-alkyl bonds, alkyl bonds, and pi-Sigma bonds. Therefore, it was suggested that Calotropis gigantia latex may represent a possible herbal remedy to attenuate H3N2 infections based on the above findings of the fragrance profile and docking.

Beta macrocarpa, Guss is an interesting species showing very low germination rates. The leading objectives of this work were to investigate the dormancy mechanism and to find methods to break dormancy in order to achieve rapid, uniform and high germination. Macro and micro-morphologic analyses were performed by stereo microscopy and scanning electron microscopy showed two fruit coats. The yellow external coat or persistent perianth coat (PPC) was accrescent with 5 erect segments contiguous to the operculum of the seed capsule. This coat forms spongy layers (50 to 300 µm thick) that could be eliminated manually. The narrow internal coat or pericarp or achene coat (AC) forms woody joined seed capsules, each presenting a pressed operculum that cannot be manually opened. This coat was not adherent to seeds and was composed of compressed cells (50 to 200 µm thick) which form pockets for salt cristal. Seeds were lentiform (1 to 2 mm diameter and 0.5 to 0.8 mm thick) and highly fragile. The embryo was whitish surrounded peripherally by the perisperm with two highly developed cotyledons and radical. Polyphenol concentrations in both coats showed that after 4 months of collection, total polyphenol concentrations were 4-fold higher in the pericarp than in the persistent perianth. However, after one year, this parameter decreases significantly in the pericarp, whereas, it increases to a larger extent in the perianth. Different germination tests indicated that the pericarp provides a chemical and a physical resistance to seed germination during the first 4 months of the experiment after collection. The chemical dormancy was released to higher levels of total polyphenol compounds that inhibited seed germination and seedling growth. However, the physical dormancy was associated with the hardness of this intern coat which caused a mechanical resistance to radicle emergence. After one year of storage, total polyphenol pericarp concentration decreased notably, and chemical resistance disappeared, whereas the physical one persisted. Consequently, one year of storage pericarp removal is sufficient to break this exogenous dormancy.