Freeze-thawing plays a vital role in enhancing materials in medicines. Here, we describe the F-T process of synthesis of Poly (vinyl alcol)- Methylene blue single strand- Mxene (PVA–MB-ssDNA –Mxene), which may be effective for gen delivery applications. The PVA –MB-ssDNA –Mxene hydrogel was formed using 1,3,5 consecutive cycles. We also demonstrated that PVA –MB-ssDNA –Mxene hydrogel can be formed by the affection of DNA with PVA and the MXene network. The F-T process shows the new intra molecular bond of PVA-PVA, compared to the non F-T hydrogel which formed by a biologic crosslinking as MB-ssDNA. Scanning electron microscopy reported that the microstructure. The differential scan shows three endothermic peaks at 70, 180, and 300 ℃ for water loss and decomposition. The swelling behavior rapidly increased due to the PVA chains in the F-T methods and then became stable. With a high concentration of MB-DNA, the tensile strength was slightly high, and the swelling behavior was low. Our results indicated that the PVA –MB-ssDNA –Mxene hydrogel using F-T process would have more suitable structural features as gene hydrogel carrier which need greater mechanical strength and stability in body analyses.

Cyber-physical Systems (CPS) have revolutionized urban transportation worldwide, but their implementation in developing countries faces significant challenges, including infrastructure modernization, resource constraints, and varying internet accessibility. This paper proposes a methodological framework for optimizing the implementation of Cyber-Physical Urban Mobility Systems (CPUMS) tailored to improve the quality of life in developing countries. Central to this framework is the Dependency Structure Matrix (DSM) approach, augmented with advanced artificial intelligence techniques. The DSM facilitates the visualization and integration of CPUMS components, while statistical and multivariate analysis tool such as Principal Component Analysis (PCA) and artificial intelligence methods such as K-means clustering enhance complex system the analysis and optimization of complex system decisions. These techniques enable engineers and urban planners to design modular and integrated CPUMS components that are crucial for efficient, and sustainable urban mobility solutions. The interdisciplinary approach addresses local challenges and streamlines the design process, fostering economic development and technological innovation. Using DSM and advanced artificial intelligence, this research aims to optimize CPS-based urban mobility solutions, by identifying critical outliers for targeted management and system optimization.

This article aims to explore the training model of preschool physical education teachers based on the theory of "space, capital, and habits". Preschool physical education plays an important role in the development of children's physical fitness and cognitive abilities. This article first introduces the theory of "space, capital, and habits", including its definition and core concepts, as well as its application value in teacher training. Subsequently, a training model for preschool physical education teachers based on this theory was proposed, which includes three elements: space, capital, and habits. In terms of space, it is emphasized to create an environment and place conducive to the professional development of preschool physical education teachers, such as the construction of training institutions and internship bases, and the support of teaching environment and resources. In terms of capital, emphasis is placed on cultivating the professional knowledge and abilities of preschool physical education teachers, including curriculum design and teaching methods, teacher team construction, and professional development mechanisms. In terms of habits, emphasis is placed on cultivating the professional literacy and educational attitude of preschool physical education teachers, including practical links and social participation, evaluation and feedback mechanisms. This training model aims to improve the quality and effectiveness of preschool physical education teacher training, and provide theoretical guidance and practical suggestions for preschool physical education teacher training.

This study conducted a systematic review of the existing literature on rhythmic gymnastics. Through searching databases such as PubMed, Web of Science, and Scopus, 37 out of 2319 articles were selected, covering training and physical fitness, nutrition and metabolism, as well as sports injuries and rehabilitation. The findings revealed that: (1) Core physical training significantly enhanced athletes’ performance; (2) Inadequate nutritional intake was prevalent; (3) The incidence of sports injuries was high, particularly those resulting from overtraining. The conclusion emphasizes the need to enhance strength training, optimize nutritional management, and further investigate injury prevention and rehabilitation measures to enhance athletes’ performance and health status.

Taking learning as the basis, practice as the path, and competition as the promotion. In the process of coordination and unity of learning-practice-competition, it can promote students' learning motivation, strengthen students' practice motivation, and promote students' active performance in competition activities. Under the influence of positive self-efficacy performance, active sense of achievement, etc., it can promote students' interest and experience in sports activities, strengthen students' learning effects, and promote the active construction of high-quality sports classrooms in junior high schools. Next, this article will discuss the effective construction of a high-quality junior high school sports classroom under the background of the integration of "learning-practice-competition" based on its own junior high school physical education teaching practice.