Introduction: Growth, yield and quality of okra (Abelmoschus esculentus (L.) Moench) are related to fertilizer application, being nitrogen (N) the most outstanding, due to its direct relationship with photosynthesis and vegetative growth of the plant. Objective: The objective was to evaluate the agronomic and productivity characteristics of okra as a function of N dose. Materials and methods: The study was conducted at the experimental area of Campus Gurupi, the Universidad Federal de Tocantins (UFT), Brazil, in two planting periods (autumn/winter and spring/summer). The experimental design used was randomized block design (RBD) with six treatments (50, 100, 150, 150, 200 and 250 kg N ha-1) and four replications. Urea was used as a source of N. The characteristics evaluated were: productivity, average fruit mass, height and plant chlorophyll index. Results: Productivity and plant height were superior in the fall/winter crop. Mean fruit mass and chlorophyll index were not influenced by planting time. For productivity, a linear response was obtained with increasing dose up to the limit of the N dose used (250 kg ha-1), with a mean value higher than 14 t of fruit. Mean mass and plant height responded linearly to increasing N dose. Nitrogen affected the chlorophyll index, with maximum values of 45.96 and 47.19, observed in the two evaluation periods. Conclusion: Planting time and N content in the soil interacted with plant height, being favorable in the period without precipitation. N influenced all the characteristics, demonstrating the importance of nitrogen fertilization in the development of okra plants.
Objective: To study the changes of growth, physiological and absorption characteristics of Pinus bungeana under ozone (O3) stress, to elucidate the correlations among the indicators, and to determine its degree of response to O3. Methods: The growth, physiological characteristics and O3 uptake capacity of Pinus bungeana seedlings were measured in an open-top O3 fumigation manual control experiment with three concentration gradients (NF: normal atmospheric O3 concentration, NF40: normal atmospheric O3 concentration plus 40 nmlol/mol; NF80: normal atmospheric O3 concentration plus 80 nmol/mol), and the relationships between the characteristics of Pinus bungeana under different O3 concentrations were investigated with correlation analysis, redundancy analysis and analysis of variance. Results: (1) Plant height growth (ΔH), diameter growth at 50 cm (ΔDBH), stomatal size (S), stomatal density (M), stomatal opening (K), stomatal conductance (Gs), net photosynthetic rate (Pn), transpiration rate (Et), water use efficiency (WUE), maximum photochemical efficiency (Fv/Fm), chlorophyll content (CHL), whole tree water consumption (W), and O3 uptake rate () all decreased with the increase of O3 concentration; while intercellular CO2 concentration () and relative conductivity (L) increased with the increase of O3 concentration; (2) growth indicators of Pinus bungeana under O3 stress (ΔH, ΔDBH) were the most correlated with O3 uptake status (, W), followed by photosynthetic indicators (, WUE, ,, ) and growth indicators (ΔH, ΔDBH) and stomatal characteristics (K, M, S) under O3 stress, some physiological indicators (L, ) were relatively weakly correlated with photosynthesis (, WUE,,, ) and stomatal (K, M, S); (3) all the indicators of Pinus bungeana were significantly different under O3 treatments of NF and NF80 (P < 0.05), ΔH, ΔDBH, M, CHL, , , W and were most significantly different under NF and NF40 treatments, and K, S, WUE, , , , L were more significantly different under NF40 and NF80 treatments. Conclusion: The experiment proved that the growth of Pinus bungeana was slowed, photosynthetic capacity was reduced, and the absorption capacity of O3 was further reduced by long-term exposure to high concentration of O3. The growth of Pinus bungeana was most correlated with the changes of O3 absorption characteristics, and the stomatal characteristics were most correlated with photosynthetic physiological characteristics, and the reduction of photosynthetic capacity etc. further led to the curtailment of its growth.
The purpose of this study is to predict the frequency of mortality from urban traffic injuries for the most vulnerable road users before, during and after the confinement caused by COVID-19 in Santiago de Cali, Colombia. Descriptive statistical methods were applied to the frequency of traffic crash frequency to identify vulnerable road users. Spatial georeferencing was carried out to analyze the distribution of road crashes in the three moments, before, during, and after confinement, subsequently, the behavior of the most vulnerable road users at those three moments was predicted within the framework of the probabilistic random walk. The statistical results showed that the most vulnerable road user was the cyclist, followed by motorcyclist, motorcycle passenger, and pedestrian. Spatial georeferencing between the years 2019 and 2020 showed a change in the behavior of the crash density, while in 2021 a trend like the distribution of 2019 was observed. The predictions of the daily crash frequencies of these road users in the three moments were very close to the reported crash frequency. The predictions were strengthened by considering a descriptive analysis of a range of values that may indicate the possibility of underreporting in cases registered in the city’s official agency. These results provide new elements for policy makers to develop and implement preventive measures, allocate emergency resources, analyze the establishment of policies, plans and strategies aimed at the prevention and control of crashes due to traffic injuries in the face of extraordinary situations such as the COVID-19 pandemic or other similar events.
To investigate the possible role of arbuscular mycrrhizal fungi (AMF) in alleviating the negative effects of salinity on Stevia rebaudiana (Bert.), the regenerated plantlets in tissue culture was transferred to pots in greenhouse and inoculated with Glomus intraradices. Salinity caused a significant decrease in chlorophyll content, photosynthesis efficiency and enhanced the electrolyte leakage. The use of AMF in salt –affected plants resulted in improved all above mentioned characteristics. Hydrogen peroxide and malondialdehyde (MDA) contents increased in salt stressed plants while a reduction was observed due to AMF inoculation. CAT activity showed a significant increase up to 2 g/l and then followed by decline at 5 g/l NaCl in both AMF and non-AMF treated stevia, however, AMF inoculated plants maintained lower CAT activity at all salinity levels (2 and 5 g/l). Enhanced POX activities in salt- treated stevia plants were decreased by inoculation of plants with AMF. The addition of NaCl to stevia plants also resulted in an enhanced activity of SOD whilst, AMF plants maintained higher SOD activity at all salinity levels than those of non-AMF inoculated plants. AMF inoculation was capable of alleviating the damage caused by salinity on stevia plants by reducing oxidative stress and improving photosynthesis efficiency.
The use of autonomous weapons systems (AWS) has led to several opposing legal opinions regarding their violations of international law. The responsibility of the state, individuals, and corporations as producers, designers, and programmers is all being taken into consideration. If the decision to kill humans without “meaningful human control” is transferred to computers, it would be hard to attribute accountability for the actions of AWS to their corporations. Consequently, this means that corporate actors will enjoy impunity in all cases. The present paper indicates that the most significant problem arising from the use of AWS is the attribution of responsibility for its violation. Corporations are not subject to liability for the legitimate use of weapons under international law. The main problem with corporate responsibility, according to article 25 (4) of the Rome Statute, is that the provision only relates to individual criminal responsibility and that the ICC shall only have jurisdiction over natural persons. Nevertheless, corporations may be held accountable under aspects of international law. The paper proposes a more positive view on artificial intelligence, raising corporations’ accountability in international law by historically linking the judging of business leaders. The article identifies aiding and abetting as well as co-perpetration as the two modes of accountability under international law potentially linked to AWS. The study also explores the main ambiguity in international law relating to corporate aiding and abetting of human rights violations by presenting the confusion on determining the standards of these 2 modes of liability before the ICC and International ad doc Tribunal. Moreover, with the new age of war heavily dependent on AI and AWS, one cannot easily and precisely ascertain who must be held accountable for war crimes because of the unanticipated facts in decision-making combined with the aiding or abetting of violations of international law. International law prioritizes the goal of ending impunity for the individual and largely neglects the need to achieve the same goal for corporate complicity. In sum, progress to regulate the use of AWS by corporate actors could be enormously helpful to the cause of ending impunity.
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