This study focused on the formulation and characterization of silver nanoparticles (AgNP) functionalized with d-limonene. The nanoparticles were functionalized by phase inversion and the synthesis of the nanoparticles was performed in situ; particle size was determined by laser diffraction, zeta potential and optical colloidal stability using Multiscan 20 for a period of 24 hours at 37 °C; the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the formulated material on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Klebsiella oxytoca ATCC 700324, Enterococcus casseliflavus ATCC 700327, Escherichia coli BLEE, carbapenem-resistant Pseudomona aeruginosa were determined. The nanoparticles showed colloidal stability at a d-limonene concentration of 3.93%, silver ions at 1.61 × 10−3%, non-ionic adjuvant at 24% and ascorbic acid at 5.88%; citric acid/citrate (1:1) 0.48M for a pH of 4.5 was used as a buffer system. The formulation was classified as a polydisperse system (PD = 0.0851), with a zeta potential of −11.6 mV and average particle size of 81.5 ± 0.9 nm. A particle migration velocity of −0.199 ± 0.006 mm∙h−1, a constant transmission profile and backscattering profile with variations of 10% were evidenced, which represents a stable formulation. The nanoparticles presented an MIC and an MBC of 28 μg∙mL−1 (5.6 × 10−2% d-limonene and 4.7 × 10−5% AgNP) against all tested bacteria.
Deficiencies in postharvest technology and the attack of phytopathogens cause horticultural products, such as tomatoes to have a very short shelf life. In addition to the economic damage, this can also have negative effects on health and the environment. The objective of this work is to evaluate an active coating of sodium alginate in combination with eugenol-loaded polymeric nanocapsules (AL-NP-EUG) to improve the shelf life of tomato. Using the nanoprecipitation technique, NPs with a size of 171 nm, a polydispersity index of 0.113 and a zeta potential of −2.47 mV were obtained. Using the HS-SPME technique with GC-FID, an encapsulation efficiency percentage of 31.85% was determined for EUG. The shelf-life study showed that the AL-NP-EUG-treated tomatoes maintained firmness longer than those without the coating. In addition, the pathogenicity test showed that tomatoes with AL-NP-EUG showed no signs of damage caused by the phytopathogen Colletotrichum gloesporoides. It was concluded that the formulation of EUG nanoencapsulated and incorporated into the edible coating presents high potential for its application as a natural nanoconservative of fruit and vegetable products such as tomato.
In this study, the influence of sewage sludge ash (SSA) waste particle contents on the mechanical properties and interlaminar fracture toughness for mode I and mode II delamination of S-glass fiber-reinforced epoxy composites was investigated. Composite laminate specimens for tensile, flexural double-cantilever beam (DCB), and end-notched fracture (ENF) tests were prepared and tested according to ASTM standards with 5, 10, 15, and 20 wt% SSA-filled S-glass/epoxy composites. Property improvement reasons were explained based on optical and scanning electron microscopy. The highest improvement in tensile and flexural strength was obtained with a 10 wt% content of SSA. The highest mode I and mode II interlaminar fracture toughness’s were obtained with 15 wt% content of SSA. The mode I and mode II interlaminar fracture toughness improved by 33% and 63.6%, respectively, compared to the composite without SSA.
In this study, ‘Xinli No. 3’, ‘Shengli rootstock’, ‘Shenli rootstock’ and ‘Shengzhen No. 1’ were used as rootstock, and ‘Jinchun No. 39’ cucumber was used as scion to study the effects of different rootstock on the yield and quality of grafted cucumber, and to select high quality rootstock suitable for cucumber grafting. Different rootstock affected the survival rate, phenology, the height of plant, stem diameter, growth potential, yield and quality of cucumber grafting. Among them, the survival rate of ‘Shenli rootstock’ grafted cucumber is the highest, and the growth of ‘Shengzhen No. 1’ grafted cucumber is relatively the strongest. There was no significant difference in fruit tuber, melon edge, thorn color and pulp crispness between self-rooted seedling (CK) and each rootstock grafting combination. The average yield of ‘Xinli No. 3’ grafted cucumber plot was not significantly different from that of self-rooted seedlings (CK). The length of ‘Shenli rootstock’ and ‘Shengli rootstock’ grafted cucumber was significantly higher than that of self-rooted seedlings (CK), and the length of ‘Shengzhen No. 1’ Grafted Cucumber was significantly higher than that of self-rooted seedlings (CK). The contents of vitamin C and soluble protein of ‘Shengli rootstock’, ‘Shenli rootstock’ and ‘Shengzhen No. 1’ grafted cucumber were significantly higher than those of self-rooted seedlings (CK), and the contents of soluble sugar were lower than those of self-rooted seedlings (CK). Therefore, ‘Shengzhen No. 1’ and ‘Jinchun No. 39’ have strong compatibility with cucumber. As rootstocks, the grafted cucumber plants not only have strong growth potential and high yield, but also significantly increase the content of soluble protein and vitamin C.
One of the biggest environmental problems that has affected the planet is global warming, due to high concentrations of carbon (CO2), which has led to crops such as coffee being affected by climate change caused by greenhouse gases (GHG), especially by the increase in the incidence of pests and diseases. However, carbon sequestration contributes to the mitigation of GHG emissions. The objective of this work was to evaluate the carbon stored in above and below ground biomass in four six-year-old castle coffee production systems. In a trial established under a Randomized Complete Block Design (RCBD) with the treatments Coffee at free exposure (T1), Coffee-Lemon (T2), Coffee-Guamo (T3) and Coffee-Carbonero (T4), at three altitudes: below 1,550 masl, between 1,550 and 2,000 masl and above 2,000 masl. Data were collected corresponding to the stem diameters of coffee seedlings and shade trees with which allometric equations were applied to obtain the carbon variables in the aerial biomass and root and the carbon variables in leaf litter and soil obtained from their dry matter. Highly significant differences were obtained in the four treatments evaluated, with T4 being the one that obtained the highest carbon concentration both in soil biomass with 100.14 t ha-1 and in aerial biomass with 190.42 t ha-1.
Banana macropropagation in a thermal chamber is an economical technology, effective as a phytosanitary cleaning method, and efficient to enhance seedling production. The objective of this work was to evaluate the effects of corm size (CS) and benzylaminopurine (BAP) on plantain cv. Barraganete seedling proliferation in two propagation environments (PE). The treatments consisted of two levels of BAP (with and without BAP), three CS (2 ± 0.5, 4 ± 0.5 and 6 ± 0.5 kg) and two PE (thermal chamber and raised bed). The variables evaluated were sprouting time (days), multiplication rate (MT) per unit (seedlings per corm) and area (seedlings per m2). Sprouting time was significantly influenced (p < 0.05) by the PE, where the thermal chamber advanced shoot emergence by 12 days, with respect to the raised bed. MT of seedlings per corm and m2, were significantly influenced (p < 0.05) by BAP × AP and TC × AP interactions, where the highest seedling production per corm occurred inside thermal chamber with BAP and 6 ± 0.5 kg corms, while seedling production per m2 was higher with 2 ± 0.5 kg corms under the same thermal chamber conditions and with BAP. The main effects results reported that with BAP there were 30 and 31% increases in MT per corm and per m2, respectively, relative to the treatment without BAP. Within the thermal chamber the MT per corm and per m2 increased by 44% relative to the raised bed. Regarding the effect of CS, larger corms achieved higher individual MT, while smaller corms achieved higher MT per area. The use of a thermal chamber and BAP is recommended for mass production of banana seedlings through macropropagation.
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