Cysteine is one of the body’s essential amino acids to build proteins. For the early diagnosis of a number of diseases and biological issues, L-cysteine (L-Cys) is essential. Our study presents an electrochemical sensor that detects L-cysteine by immobilizing the horseradish peroxidase (HRP) enzyme on a reduced graphene oxide (GCE) modified glassy carbon electrode. The morphologies and chemical compositions of synthesized materials were examined using Fourier transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FESEM). The modified electrode’s electrochemical behavior was investigated using cyclic voltammetry (CV). Cyclic voltammetry demonstrated HRP/rGO/GCE has better electrocatalytic activity than bare GCE in the oxidation of L-cysteine oxidation in a solution of acetate buffer. The electrochemical sensor had a broad linear range of 0 µM to 1 mM, a 0.32 µM detection limit, and a sensitivity of 6.08 μA μM⁻¹ cm⁻². The developed sensor was successfully used for the L-cysteine detection in a real blood sample with good results.

Vegetable production is an important sector of economy for farmers in Nepal. The analysis was carried out to explore the trends in vegetable production sector in Nepal along with the recent trend of some major vegetables in terms of area, production and yield. The time series data from 1977/78 to 2016/17 (40 years) of vegetables production and 5 years data (2011/12 - 2015/16) of major vegetables were collected from reliable source and analysis was done through Microsoft Excel. The results show that between 1977/78 and 2016/17 the area under vegetables cultivation has jumped by 222.8% while production is increased by 728.21% and productivity is increased by 156.6% during this course. The result also reveals that during the period of 5 years (2011/12 - 2015/16), solanaceous and cruciferous vegetables has an increasing trend in area, production and yield except for the area under cultivation for eggplant (declined by 5.2%) and for radish (declined by 6.0%) respectively while cucurbitaceous vegetables has increasing trend in area and production but an declining trend in yield except for the yield of cucumber (increased by 15.8%). However, the trend of other major vegetables is seen highly fluctuating over the years. 

India’s economic growth is of significant interest due to its expanding Gross Domestic Product (GDP) and global market influence. This study investigates the interplay between production, trade, carbon dioxide (CO₂) emissions, and economic growth in India using Granger causality analysis. Also, the data from 1994 to 2023 were analyzed to explore the relationships among these variables. The results reveal strong positive correlations among production, trade, CO₂ emissions, and GDP, with production showing significant associations with export, import, and GDP. Co-integration tests confirm the presence of a long-term relationship among the variables, suggesting their interconnectedness in shaping India’s economic landscape. Regression analysis indicates that production, export, import, United States (US)-India trade, manufacturing cost of energy, and CO₂ emissions significantly impact GDP. Moreover, the Vector Error Correction Model (VECM) estimation reveals both short-term and long-term dynamics, highlighting the importance of understanding equilibrium and deviations in economic variables. Overall, this study contributes to a better understanding of the complex interactions driving India’s economic growth and sustainability.

The environmental issue of single-use plastic is extremely discussed due to waste accumulation and the consumption of non-renewable resources. This study aims to investigate the properties of bioplastic compared to petroleum-based plastic. Two stages of stretch blow molding were used to fabricate polyethylene terephthalate (PET) and bio-polyethylene terephthalate (Bio-PET) bottles. The shelf life extension of chili sauce paste stored in PET and Bio-PET containers with an oxygen scavenger at 45 ℃ in an accelerated condition was investigated. After twelve weeks, the chili sauce paste stored in the container bottle was observed. PET and Bio-PET bottles without oxygen scavengers were also determined as a control for comparison. The result showed that both PET and Bio-PET bottles with oxygen scavengers could prolong the quality of chili sauce paste similarly, meaning that PET could be replaced by Bio-PET as a chili sauce paste container. Other properties, such as thickness gauge, color, leak test, drop test, and close-open force of the container bottle, were also verified to check the product quality standard.

This research quantitatively examines how technology-mediated formative assessment techniques affect student learning outcomes in middle school education. The research investigates the correlation between instructors’ technology use, attitudes, and student performance in several academic disciplines using surveys and evaluations conducted with teachers and students. Results show strong positive connections between how often technology is used, the specific digital tools used, how effective technology-mediated formative assessment is judged to be, and the results of student learning. On the other hand, obstacles to implementation were shown to have a negative relationship with student accomplishment. The research emphasizes that technology-mediated formative assessment is more successful in some subjects, emphasizing the necessity to customize teaching methods for each subject’s requirements. The study revealed a positive correlation between student learning outcomes and the frequency of technology use, the types of digital tools used, and the perceived effectiveness of technology-mediated formative assessment. These results suggest ways to improve the use of technology and formative assessment in middle school instruction.