In Nigeria, deforestation has led to an unimaginable loss of genetic variation within tree populations. Regrettably, little is known about the genetic variation of many important indigenous timber species in Nigeria. More so, the specific tools to evaluate the genetic diversity of these timber species are scarce. Therefore, this study developed species-specific markers for Pterygota macrocarpa using state-of-the-art equipment. Leaf samples were collected from Akure Forest Reserve, Ondo State, Nigeria. DNA isolation, quantification, PCR amplification, gel electrophoresis, post-PCR purification, and sequencing were done following a standardized protocol. The melting temperatures (TM) of the DNA fragments range from 57.5 ℃to 60.1 ℃ for primers developed from the MatK gene and 58.7 ℃ to 60.5 ℃ for primers developed from the RuBisCo gene. The characteristics of the ten primers developed are within the range appropriate for genetic diversity assessment. These species-specific primers are therefore recommended for population evaluation of Pterygota macrocarpa in Nigeria.

Vascular access in hemodialysis is one of the pillars of success of the program. Therefore, efforts should be directed firstly to achieve the greatest number of vascular accesses of the arteriovenous fistula type, and secondly to reduce complications related to access cannulation in order to functionally preserve the access. Several strategies have been described to improve this last aspect; this article describes the use of ultrasound to improve the probability of successful cannulation in cases considered difficult by the nursing team.

In recent years, phytoremediation as a promising ecological restoration technique has emerged. Phytoremediation is a repair method that uses green plants to transfer, contain, or convert contaminants to the environment. Phytoremediation is a heavy metal, organic or radioactive element contaminated soil and water. The results show

that the use of plant absorption, volatilization, root filtration, degradation, stability and other effects, can purify soil

or water pollutants, to achieve the purpose of purifying the environment, so phytoremediation is a great potential, the development of the clean environment Pollution of green technology. The use of plants to repair contaminated soil is a cheap and durable bioremediation technique. The protection and management of Taihu Lake is an indispensable measure for the protection of Taihu Lake water, and the advantages of phytoremedry investment, low freight and

low leakage of pollutants show that its promotion has this unusual significance. This paper expounds the difference

of remediation soil between Taihu Lake Ecological Shelter Forest, and the comparison of the soil capacity of the

experimental tree species. Second, the correlation between the monitoring projects is discussed.

The national park with Chinese characteristics is the highest level of protection of a kind of natural protection, its establishment marks the park will implement the strictest ecological protection means. It is of great value to construct the utilization system of national park resources under the new natural protected area system in the new era to avoid the misunderstanding of “ecological protection only” and explore how to carry out the sustainable utilization of resources in the reform of national park system and mechanism. According to the analytic hierarchy process (AHP) and Delphi method, the evaluation framework, indicators, reference standards and weights of resource utilization under the national park system were determined in combination with the requirements of constructing the protected natural area system and the total value of resource ecosystem services (including harvest value, existence value and future value). Based on the application research of Bawangling zone of Hainan Tropical Rainforest National Park, the optimal resource utilization system in the future was proposed, and two optimization strategies of ecological adjustment of resource utilization system and construction of suitable resource utilization system were put forward.

Dust is one of the atmospheric pollutants that have adverse environmental effects and consequences. Dust fall contains particles of 100 microns or even smaller ones, which fall from the atmosphere onto the earth surface. The aim of this study is to determine the concentration of lead in dust fall samples in order to study the pollution level of this element in Zahedan, Sistan and Baluchistan Province, Iran. Therefore, sampling was carried out using 30 marble dust collectors (MDCO) for 3 months in the spring of 2015 to investigate the quantitative variation and spatial analysis of lead content in dust fall. These dust collectors were placed at 30 stations on the building roofs with a height of approximately 1.5 meters across the city. According to the results, the mean lead concentration in the spring was 90.16 mg/kg. In addition, the zoning map of lead content shows that the lowest level of lead was measured at Imam Khomeini station while the highest amount of lead appeared in Mostafa Khomeini station.