Lettuce (Lactuca sativa L.) is the main leafy vegetable grown in Brazil. Its productivity and quality are limited by the growing season, the nearby environment and the type of cultivar adopted. The objective of this work was to verify at different times of the year the best planting environment for lettuce cultivation in a semi-humid tropical climate. For this purpose, an experiment was set up in three different seasons (October–November 2014, January–March, May–July 2015). The experimental design was randomized blocks, in a 3 × 3 × 2 factorial arrangement, consisting of three seasons, three cultivars (cvs. Vera^®, Tainá^® and Rafaela^®) and two growing environments (low tunnel with beds protected with mulching consisting of soil protection with plastic fabric covering, and beds without protection or conventional cultivation) and four replicates per treatment. Plant biomass, stem length, head diameter, number of leaves per head and crop productivity were evaluated as response parameters. The results showed that the May–July period favored biomass production, head diameter and productivity. Despite the similarity between varieties, the variety Vera^® is more productive in biomass, number of leaves per head, stem length and productivity. The low tunnel planting system with mulching is adequate under the conditions evaluated for lettuce cultivation. This system in the May–July period favors a superior development in the characteristics biomass, head diameter and productivity, if compared to conventional cultivation during the October–November period.