In Nigeria, deforestation has led to an unimaginable loss of genetic variation within tree populations. Regrettably, little is known about the genetic variation of many important indigenous timber species in Nigeria. More so, the specific tools to evaluate the genetic diversity of these timber species are scarce. Therefore, this study developed species-specific markers for Pterygota macrocarpa using state-of-the-art equipment. Leaf samples were collected from Akure Forest Reserve, Ondo State, Nigeria. DNA isolation, quantification, PCR amplification, gel electrophoresis, post-PCR purification, and sequencing were done following a standardized protocol. The melting temperatures (TM) of the DNA fragments range from 57.5 ℃to 60.1 ℃ for primers developed from the MatK gene and 58.7 ℃ to 60.5 ℃ for primers developed from the RuBisCo gene. The characteristics of the ten primers developed are within the range appropriate for genetic diversity assessment. These species-specific primers are therefore recommended for population evaluation of Pterygota macrocarpa in Nigeria.

An appraisal of the groundwater potential of Alex Ekwueme Federal University Ndufu Alike was carried out by integrating datasets from geology, geographic information system and electrical resistivity survey of the area. The study area is underlain by the Asu River group of Albian age. The Asu River Group in the Southern Benue Trough comprises of Shales, Limestones and Sandstone lenses of the Abakaliki Formation in Abakaliki and Ikwo areas. The shales are generally weathered, fissile, thinly laminated and highly fractured and varies between greyish brown to pinkish red in colour. Twenty (20) Vertical Electrical Sounding data were acquired using SAS 1000 ABEM Terrameter and processed to obtain layer parameters for the study area. A maximum current electrode spacing (AB) of 300 meters was used for data acquisition. Computer aided iterative modelling using IPI2 Win was used to determine layer parameters. In-situ Hydraulic Conductivity measurements at seven parametric locations within the study area were conducted and integrated with Electrical Resistivity measurements to determine aquifer parameters (e.g., Hydraulic conductivity and Transmissivity) in real time. This technique reduces the attendant huge costs associated with pumping tests and timelines required to carry out the technique. Accurate delineation of aquifer parameters and geometries will aid water resource planners and developers on favourable areas to site boreholes in the area. Several correlative cross-sections were generated from the interpreted results and used to assess the groundwater potential of the study area. Results show that the resistivity of the the aquifer ranges from 7.3 Wm–530 Wm while depth to water ranges from 11.4 m to 55.3 m. Aquifer thicknesses range from 8.7 m at VES 5 to 36.3 m at VES 6 locations. Hydraulic conductivity ranges from 1.55 m/day at VES 15.18, and 19 locations to 9.8 m/day at VES 3 and 4 locations respectively. Transmissivity varies from 17.48 m²/day at VES 19 to 98 m²/day at VES 3 locations respectively. Areas with relatively high transmissivities coupled with good aquifer thicknesses should be the target of water resource planners and developers when proposing sites for drilling productive boreholes within Alex Ekwueme federal University Ndufu Alike.

Phytochemical and antioxidant analysis of some varieties of Capsicum was evaluated. Mature Capsicum varieties were collected across the State. The seeds were removed, sun-dried for 3 days, stored for 2 weeks at 15 ºC–25 ºC in polythene bags before planting. Saponins, tannins, flavonoids, alkaloids and cardiac glycosides were present in abundant, moderate and trace amounts. Combined anthraquinones were absent in all varieties. Yellow (0.810 ± 0.0006 µg/mL), red long dry (0.211 ± 0.0006 µg/mL) and round peppers (2.527 ± 0.0003 µg/mL) had the largest values for total phenol, flavonoids and tannins. Shombo and yellow peppers had the largest (0.270 ± 0.002 µg/mL) and least (0.102 ± 0.001 µg/mL) capsaicin content. The antioxidant activities varied across the varieties. At 100 µg/mL of methanol, yellow (45%) and round peppers (45%) had largest mean absorbances for 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Radical Scavenging Activity while sub-shombo pepper (23%) had the least. For Ferric Reducing Antioxidant Power (FRAP), yellow (0.63 ± 0.001 µg/mL) and sub-shombo peppers (0.55 ± 0.001µg/mL) had the largest and least values at 100 µg/mL of methanol. At 100 µg/mL of methanol, red long dry (0.112 ± 0.001) and shombo peppers (0.101 ± 0.001) had the largest and least values for the nitric oxide scavenging activity. This study shows that Capsicum varieties exhibit bioactive componds similarities and variations with implications in hybridization, taxonomy and conservation.

Studies to evaluate the response of passion fruit seedlings in terms of emergence, nursery, and early field growth to growing media and mulching were carried out at the Teaching and Research Farm of Joseph Sarwuan Tarka University Makurdi between July and December 2018. Treatments consisted of five media, composted from readily available substrates. The five nursery media were; medium 1:1:2:3 (SB) composed of top soil + poultry manure + river sand; medium 2:1:2:3 (RHB) – rice hull + poultry manure + river sand; medium 3:2:3:1 (RHB) – rice hull + poultry manure + river sand; medium 4:1:4:3 (SDB) – sawdust + poultry manure + river sand and medium 5:1:2:3 (SDB) – sawdust + poultry manure + river sand. For the nursery experiment, treatments were the five potting media, while the field trial was a 5 × 2 factorial arrangement consisting of the five growing media and mulching status (mulch and no mulch). In both cases, treatments were laid out in randomized designs that were replicated three times. Results showed that there were no significant differences in all the emergence traits evaluated. However, medium M5 (sawdust based) showed superior performance in most of the seedling characters evaluated. Under field conditions, the sawdust based media (M4 and M5) gave the best growth of passion fruit seedlings compared to the other potting media. Application of mulch, however, did not elicit any significant response in plant growth. It is therefore conclusive that sawdust based growing media could be used to produce high quality passion fruit seedlings with the prospect of excellent performance under field conditions.

Malaria is a mosquito-borne infectious disease that affects humans and poses a severe public health problem. Nigeria has the highest number of global cases. Geospatial technology has been widely used to study the risks and factors associated with malaria hazards. The present study is conducted in Ibadan, Oyo State, Nigeria. The objective of this study is to map out areas that are at high risk of the prevalence of malaria by considering a good number of factors as criteria that determine the spread of malaria within Ibadan using open-source and Landsat remote sensing data and further analysis in GIS-based multi-criteria evaluation (MCE). This study considered factors like climate, environmental, socio-economic, and proximity to health centers as criteria for mapping malaria risk. The MCE used a weighted overlay of the factors to produce an element at-risk map, a malaria hazard map, and a vulnerability map. These maps were overlaid to produce the final malaria risk map, which showed that 72% of Ibadan has a risk of malaria prevalence. Identification and delineation of risk areas in Ibadan would help policymakers and decision-makers mitigate the hazards and improve the health status of the state.

Building cooling load depends on heat gains from the outside environment. Appropriate orientation and masonry materials play vital roles in the reduction of overall thermal loads buildings. A net-zero energy building performance has been analyzed in order to ascertain the optimum orientation and wall material properties, under the climatic conditions of Owerri, Nigeria. Standard cooling load estimation techniques were employed for the determination of the diurnal interior load variations in a building incorporating renewable energy as the major energy source, and compared with the situation in a conventionally powered building. The results show a 19.28% reduction in the building’s cooling load when brick masonry was used for the wall construction. It was observed that a higher heat gain occurred when the building faced the East-West direction than when it was oriented in the North-South direction. Significant diurnal cooling loads variation as a result of radiation through the windows was also observed, with the east facing windows contributing significantly higher loads during the morning hours while the west facing windows contributed higher amounts in the evening. The economic analysis of the net-zero energy building showed an 11.63% reduction in energy cost compared to the conventional building, with a 7-year payback period for the use of Solar PV systems. Therefore, the concept of net-zero energy building will not only help in energy conservation, but also in cost savings, and the reduction of carbon footprint in the built environment.