The study evaluated the aseptic establishment of Monstera acuminata Koch and Monstera deliciosa Liebm (Araceae) from leaves and the induction of in vitro organogenesis of M. acuminata K. from stem discs of young shoots. For this purpose, different disinfection protocols were applied to mature leaves and young shoots, from which leaf explants of approximately 1 cm² and stem discs of approximately 1 mm thickness were extracted. The explants were established in semi-solid media with different hormone treatments during the aseptic establishment stage and induction of organogenesis. Disinfection with 3% sodium hypochlorite (NaClO) for 20 min and 50% Murashige and Skoog^[1] medium with plant tissue culture preservative (PPM) favored less oxidation in leaf explants of both species. All explants of M. deliciosa in both treatments grown in PPM-added medium and at different disinfection protocols survived, showed no contamination and more than 80% retained cellular activity up to 49 days of culture age. At 35 days of culture, with disinfection in Tween-20 + 20% ethanol + 2.5% NaClO, and seeding of explants in MS medium added with 1 mg/L of BAP, 0.5 mg/L of AIA and 0.1 mg/L of ANA, seven new shoots of stem discs were induced. Monstera deliciosa was more adaptable to in vitro conditions. Advances in aseptic establishment and induction of organogenesis in native Araceae for wicker production are the basis for ex situ conservation of local populations.

This study aims to apply mathematical modelling methods focusing on the fishing songs of Poyang Lake for its conservation and digital reform. Through the principles of abstraction, model building and parameter estimation of mathematical modelling, we will quantitatively analyse the efficiency of cultural heritage and the degree of influence of digital reform. Specific methods include time series analysis, data mining and optimisation models. These tools will provide theoretical support and quantify the complexity of the problem by introducing corresponding mathematical models and formulas.

Payment for forest ecosystem services (PFES) policy is a prevalent strategy designed to establish a marketplace where users compensate providers for forest ecosystem services. This research endeavours to scrutinise the impact of PFES on households’ perceptions of forest values and their behaviour towards forest conservation, in conjunction with their socio-economic circumstances and their communal involvement in forest management. By incorporating the social-ecological system framework and the theory of human behaviours in environmental conservation, this study employs a structural equations model to analyse the factors influencing individuals’ perceptions and behaviours towards forest conservation. The findings indicate that the payment of PFES significantly increases forest protection behaviour at the household level and has achieved partial success in activating community mechanisms to guide human behaviour towards forest conservation. Furthermore, it has effectively leveraged the role of state-led social organisations to alter local individuals’ perceptions and behaviours towards forest protection.

Knowledge of the state of fragmentation and transformation of a forested landscape is crucial for proper planning and biodiversity conservation. Chile is one of the world’s biodiversity hotspots; within it is the Nahuelbuta mountain range, which is considered an area of high biodiversity value and intense anthropic pressure. Despite this, there is no precise information on the degree of transformation of its landscape and its conservation status. The objective of this work was to evaluate the state of the landscape and the spatio-temporal changes of the native forests in this mountain range. Using Landsat images from 1986 and 2011, thematic maps of land use were generated. A 33% loss of native forest in 25 years was observed, mainly associated to the substitution by forest plantations. Changes in the spatial patterns of land cover and land use reveal a profound transformation of the landscape and advanced fragmentation of forests. We discuss how these patterns of change threaten the persistence of several endemic species at high risk of extinction. If these anthropogenic processes continue, these species could face an increased risk of extinction.

Taking the west slope of Cangshan Mountain in Yangbi County, Dali as the research site, on the basis of investigating the local natural geographical conditions, topography and biodiversity status of Cangshan Mountain, the CAP protection action planning method was adopted, and the priority protection objects were determined to be native forest vegetation, rare and endangered flora and fauna, alpine vertical ecosystems, hard-leaf evergreen broad-leaved forests and cold-tempered coniferous forests; The main threat factors were commercial collection, tourism development and overgrazing. Biodiversity conservation on the western slope of Cangshan Mountain should take species as “point”, regional boundary as “line”, ecosystem and landscape system as “plane”, so as to realize the overall planning structure system combining “point—line—plane”, which can be divided into conservation core area, buffer zone and experimental area. The results can provide a reference for biodiversity conservation on the western slope of Cangshan Mountain.

The Nevado de Toluca Flora and Fauna Protection Area presents a constant fragmentation of its forests. The objective of the research was to identify the processes of forest deterioration and the role of local stakeholders in its conservation. Geographic information systems were used as a basis for the generation of thematic maps, in addition to the application of a flow diagram that defines the problems of the forest and another that describes and analyzes them for the search of solutions. The results show that the main factors affecting deterioration are forest fires, immoderate logging, pests and diseases. Finally, strategies and scenarios for forest management are proposed based on the articulation of local stakeholders.