With the development of higher education and socio-economic changes, the senior undergraduate program in applied chemistry at regional teacher training colleges and universities is facing unprecedented challenges and opportunities. This paper provides an in-depth discussion of the development status of the program in the current environment, such as the quality of education, teaching resources, and student employment. At the same time, the paper also analyzes the external environmental changes, educational reform and cooperation with industry, and puts forward a series of practical and innovative strategies.