The most crucial factor in producing papaya seedlings successfully is seed germination. The purpose of this study was to investigate the influence of seed priming with growing media on seed germination and seedling growth of papaya from October to December 2022. The experimental treatments included three seed priming treatments: T₀ = control (no seed priming treatments), T₁ = GA₃ (100 ppm), and T₂ = KNO₃ (1%), and four growing media, viz., M₁ = soil + vermicompost (1:1), M₂ = soil + cowdung (1:1), M₃ = soil + cocopeat + vermicompost (1:1:1), and M₄ = soil + cocopeat + cowdung (1:1:1). The treatments showed a significant effect on different parameters such as germination percentage, days to germination, survival percentage, chlorophyll content, seed vigor index, shoot, and root length. GA₃ treated seedlings performed better than non-GA₃-treated seedlings. Among the growing media, M₃ showed the best for seed germination and other growth attributes compared to other growing media. In terms of interaction effects, T₁M₃ showed the highest performance for germination percentage (84.33%), survival percentage (91.0%), and chlorophyll content (44.26%). T₁M₃ also showed the highest seed vigor index, shoot and root growth, and plant biomass. As a result, the combination of GA₃ and growing media containing soil + cocopeat + vermicompost was shown to be the most favorable for papaya seed germination and seedling growth.

Among major global threats to papaya cultivation, papaya ringspot virus (PRSV) is the most challenging one. In the absence of any PRSV resistant commercial papaya cultivar, PRSV management is restricted to minimizing yield losses. ICAR-Indian Agricultural Research Institute, Regional Station, Pune has developed PRSV tolerant dioecious papaya lines, Pune Selection (PS)-1, PS-2, PS-3 and PS-5. Being dioecious these lines have limited acceptability among farmers. Gynodioecious population from these lines were developed and characterized. They are numbered PS-1-1, PS-2-1, PS-3-1 and PS-5-1. These lines were characterized against prevailing commercial gynodioecious cultivar, Red Lady, for five generations. The average plant height of PS-2-1 and PS-5-1 (183 cm) was more than Red Lady (158 cm), however, stem girth of all lines was lesser than Red Lady. The fruiting height of all lines was less than Red Lady (87 cm). Length of the fruiting column of all lines was more than Red Lady (37 cm), except in PS-1-1. Fruit yield of all lines was more than Red Lady (16 kg/plant). Intensity of PRSV infection in Red Lady (48%) was considerably more than all lines. These lines can be used for developing PRSV tolerant gynodioecious papaya variety.