Among major global threats to papaya cultivation, papaya ringspot virus (PRSV) is the most challenging one. In the absence of any PRSV resistant commercial papaya cultivar, PRSV management is restricted to minimizing yield losses. ICAR-Indian Agricultural Research Institute, Regional Station, Pune has developed PRSV tolerant dioecious papaya lines, Pune Selection (PS)-1, PS-2, PS-3 and PS-5. Being dioecious these lines have limited acceptability among farmers. Gynodioecious population from these lines were developed and characterized. They are numbered PS-1-1, PS-2-1, PS-3-1 and PS-5-1. These lines were characterized against prevailing commercial gynodioecious cultivar, Red Lady, for five generations. The average plant height of PS-2-1 and PS-5-1 (183 cm) was more than Red Lady (158 cm), however, stem girth of all lines was lesser than Red Lady. The fruiting height of all lines was less than Red Lady (87 cm). Length of the fruiting column of all lines was more than Red Lady (37 cm), except in PS-1-1. Fruit yield of all lines was more than Red Lady (16 kg/plant). Intensity of PRSV infection in Red Lady (48%) was considerably more than all lines. These lines can be used for developing PRSV tolerant gynodioecious papaya variety.