In order to seek management alternatives for anthracnose caused by the fungus Colletotrichum gloeosporioides in blackberry (Rubus glaucus Benth.), at the Tibaitatá Research Center of the Colombian Agricultural Research Corporation AGROSAVIA (formerly CORPOICA), an experiment was conducted to evaluate the effect of the application of the major elements nitrogen (N), phosphorus (P), potassium (K) and calcium (Ca) on infections of the fungus C. gloeosporioides strain-52. For this purpose, a randomized complete block design was used with an arrangement of treatments in an orthogonal central composite design. To evaluate the relationship of fertilization levels and disease severity, an artificial inoculation was made on thorny blackberry stems using 0.5 cm mycelial discs at a concentration of 9.53 × 104 conidia. Observations consisted of: disease severity (S), incubation period (IP) and rate of development (r). Data analysis was done by the cluster method on the severity variable, a Pearson correlation analysis between variables, as well as a regression to estimate the effect of nutrients applied on the severity of C. gloeosporioides strain-52. The treatments were concentrated in four groups with the ranges (in parentheses) S (15.9% and 91.8%), PI (9 and 15.3) and Tr (0.0254 and 0.0468). A positive and significant correlation was observed between S and r (P < 0.001) and a negative correlation between PI with S and r (P < 0.001). By means of regression analysis, a linear model was generated that showed a reduction in disease severity with increasing N dose and an increase with the levels of P and Ca applied.

There are numerous studies reported on the usage of the sapindus emarginatus (SE) fruit in cancer and other treatments in the past few years. In this study, crude SE fruit extract was prepared and it was further used to synthesis gold nanoparticles (Au Nps). The synthesized Au Nps were left embedded in the SE fruit extract. The Au Nps embedded in the SE fruit extract (SE-Au Nps) were characterized using UV-Visiable Spectroscopy, Centrifugal Particle Size analyzer (CPS), Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). MTT assay was carried out for both SE fruit extract and SE-Au Nps on MCF7 breast cancer cell line and thus compared. The UV-Visible Absorbance for the SE-Au Nps was obtained at 543 nm. The centrifugal particle size analysis of the Au Nps embedded in SE fruit extract showed the size of the nanoparticles to be widely varying with higher fraction of particles between the size ranges of 15 to 20 nm. The morphology of the Au Nps embedded in SE fruit extract was observed using SEM. The presence of Au Nps in SE fruit extract was confirmed using FTIR. The results of the MTT assay on MCF7 breast cancer cell line proved that the % cell viability was less for SE-Au Nps than that of the SE fruit extract alone. Thus, the antiproliferative activity of the SE fruit extract was significantly enhanced by embedding it with Au Nps and it can be effectively used in therapeutic applications after further studies.

Taking the 13 years pure artificial forest Phoebe chekiangensis and heterogeneous mixed forests in Tiantong mountain, Zhejiang Province as the research object, the characteristics of stand development, tree competition differentiation, tree height/breast diameter ratio and dominant wood growth were compared and analyzed from the perspective of ecology. The results show that compared with pure forests, the growth advantages of heterogeneous mixed-age forests were significant. Average breast diameter growth of stand increased 1.8%; the growth of single plant wood accumulation increased 7.4%. The relationship between tree height and diameter showed that the high growth of Phoebe chekiangensis individuals in the heterogeneous mixed forest was significantly promoted, and the high growth of the tree was 8.4% higher than that of pure forest. 1–5 grade wood scale sizes Phoebe chekiangensis in heterogeneous mixed forests and pure forests are ranked grade 3 (43.7%) > grade 2 (26.5%) > grade 4 (15.7%) > grade 1 (12.9%) > grade 5 (1.2%); grade 3 (34.7%) > level 2 (25.6%) > level 4 (20.0%) > level 1 (18.2%) > level 5 (1.2%); the straight-diameter structure shows a normal distribution, and the degree of differentiation of pure forests is greater than that of heterogeneous forests. The dominant trees of Phoebe chekiangensis pure forest and heterogeneous forest accounted for 18.2% and 12.9% of the total number of plants respectively, providing a reserve of 51.1% and 35.4% respectively, reflecting the contribution of dominant trees caused by the self-thinning effect.

Knowledge of the presence of heavy metals in soils of agricultural areas is important to prevent their accumulation in cultivated plants. The objective of the present investigation was to evaluate the total concentrations and fractions of heavy metals Cd, Pb, Zn, Fe, Mn, Ni, Cu, Cr and Co in the tobacco-growing area of Pinar del Río, Cuba and their relationship with the physicochemical properties of soil. For the study, 59 samples of three types of soils were collected at 20 cm depth. The pseudo-total concentrations of metals in the soils are low and lower than the prevention values registered for Cuban soils. In general, the heavy metals studied present a high affinity for the most stable fractions of the soil, which means a low risk of transfer to the tobacco crop or accumulation in groundwater. The pseudo-total concentrations of heavy metals were low, below the alert values established for soils in the region. The heavy metals studied were mainly associated with the residual fraction, the second fraction with the highest association with metals was that linked to manganese and iron oxides. The principal component analysis showed that their main source is pedogenetic and that these elements are closely related to cation exchange capacity and calcium content.