The exploitation of timber has had a profound impact on tropical forest areas and their structures. This study assessed the effect of selective logging on natural regeneration and soil characteristics in post-loading bay sites at the Pra-Anum forest reserve in Ghana, West Africa. The results showed no difference in the number of species enumerated in the loading bays and the undisturbed area. More trees were observed in the RAT and RNT plots than in the undisturbed area. Relative to the RAT plot, species on the RNT and the undisturbed area were less diverse and less evenly distributed. Mean tree height, diameter, and basal area were higher in the RAT and RNT plots than in the undisturbed plots. Soil bulk density was lower in the RAT and undisturbed plot than in the RAT plot and increased with increased depth. Soil organic matter was 44% and 27% more in the undisturbed and RAT plots, respectively, than in the RNT plot and accounted for 84.75%, 83.97% and 45.33% of variations in soil bulk density, pH, and CEC. The study provides insight into the need to rehabilitate highly disturbed areas in forests, particularly the addition of topsoil on loading bays, skid trails, roads, and gaps after logging to improve the productivity of the forest soils.

Alginate-silver nanocomposites in the form of spherical beads and films were prepared using a green approach by using the aqueous extract of Ajwa date seeds. The nanocomposites were fabricated by in situ reduction and gelation by ionotropic crosslinking using calcium ions in solution. The rich phytochemicals of the date seed extract played a dual role as a reducing and stabilizing agent in the synthesis of silver nanoparticles. The formation of silver nanoparticles was studied using UV-Vis absorption spectroscopy, and a distinct surface plasmon resonance peak at 421 nm characteristic of silver nanoparticles confirmed the green synthesis of silver nanoparticles. The morphology of the nanocomposite beads and film was compact, with an even distribution of silver nanoclusters. The catalytic property of the nanocomposite beads was evaluated for the degradation of 2-nitrophenol in the presence of sodium borohydride. The degradation followed pseudo-first-order kinetics with a rate constant of 1.40 × 10⁻³ s⁻¹ at 23 ℃ and an activation energy of 18.45 kJ mol⁻¹. The thermodynamic parameters, such as changes in enthalpy and entropy, were evaluated to be 15.22 kJ mol⁻¹ and −197.50 J mol⁻¹ K⁻¹, respectively. The nanocomposite exhibited properties against three clinically important pathogens (gram-positive and gram-negative bacteria).

The H3N2 influenza virus is spiking dramatically, which is a major concern worldwide and in India. The multifunctional hetero-trimer influenza virus RNA-dependent RNA polymerase (RdRP) is involved in the generation of viral mRNA and is crucial for viral infectivity, which is directly related to the virus’s ability to survive. The goal of the current work was to use molecular docking to determine how the RdRP protein might be affected by powerful bioactive chemicals found in Calotropis gigantia latex. By applying CB-dock 2 analysis and 2D interactions, an in-silico docking study was conducted using a GC-FID (gas chromatography with flame-ionization detection) based composition profile. Tocospiro A (15%), Amyrin (7%), and Gombasterol A were found by GC-FID to be the main phytocompounds in the latex of Calotropis gigantia. The docking result showed that ligands were effectively bound to RdRP. According to interaction studies, RdRP/ligand complexes create hydrogen bonds, van der Waals forces, pi-alkyl bonds, alkyl bonds, and pi-Sigma bonds. Therefore, it was suggested that Calotropis gigantia latex may represent a possible herbal remedy to attenuate H3N2 infections based on the above findings of the fragrance profile and docking.

According to the World Health Organization (WHO), breast cancer is among the most common cancers worldwide. Most of the anticancer agents have been showing a variety of side effects. Recently, bacterial proteins have been investigated as promising anticancer agents. Azurin is a bacterial cupredoxin protein secreted from Pseudomonas aeruginosa and has been reported as a potent multi-targeting anticancer agent, which makes it an appropriate candidate for drug delivery. Azurin may be delivered to cancer cells using different carriers like polymeric micro and nanoparticles. In the present study, azurin was extracted from the bacterial host and loaded into chitosan particles. Then its effect on MCF-7 cell line was investigated. Chitosan-azurin particles were made using the ion gelation method. Results showed that chitosan-azurin particles are about 200 nm, and the loading of the protein in particles did not affect its integrity. The MTT assay showed a significant reduction in cell viability in azurin and chitosan-azurin-treated cells. The toxicity level after 5 days was 63.78% and 82.53% for free azurin and chitosan-azurin-treated cells, respectively. It seems using an appropriate carrier system for anticancer proteins like azurin is a promising tool for developing low side effect anticancer agents.

Species of the Moraceae family are of great economic, medicinal and ecological importance in Amazonia. However, there are few studies on their diversity and population dynamics in residual forests. The objective was to determine the composition, structure and ecological importance of Moraceae in a residual forest. The applied method was descriptive and consisted of establishing 16 plots of 20 m × 50 m (0.10 ha), in a residual forest of the Alexánder von Humboldt substation of the National Institute of Agrarian Innovation-INIA, Pucallpa, department of Ucayali, where individuals of arboreal or hemi-epiphytic habit, with DBH ≥ 2.50 cm, were evaluated. The floristic composition was represented by 33 species, distributed in 12 genera; five species not recorded for Ucayali were found. Structurally, the family was represented by 138 individuals/ha with a horizontal distribution similar to an irregular inverted “J”. However, there were different horizontal structures among species. It was determined that 85% of the species were in diameter class I (2.50 to 9.99 cm), being the most abundant Pseudolmedia laevis (Ruiz & Pav.) J.F. Macbr. (41.88 individuals/ha); and the most dominant were Brosimum utile (Kunth) Oken (1.71 m²∕ha) and Brosimum alicastrum subsp. bolivarense (Pittier) C.C.Berg (0.90 m²/ha). Likewise, P. laevis and B. utile were the most ecologically important. The information from the present research will allow the establishment of a baseline, which can be used to propose the management of Moraceae in residual forests in the same study area.