The WRKY gene family plays a very diverse role in plant growth and development. These genes contained an evolutionarily conserved WRKY DNA binding domain, which shows functional diversity and extensive expansion of the gene family. In this study, we conducted a genome-wide comparative analysis to investigate the evolutionary aspects of the WRKY gene family across various plant species and revealed significant expansion and diversification ranging from aquatic green algae to terrestrial plants. Phylogeny reconstruction of WRKY genes was performed using the Maximum Likelihood (ML) method; the genes were grouped into seven different clades and further classified into algae, bryophytes, pteridophytes, dicotyledons, and monocotyledons subgroups. Furthermore, duplication analysis showed that the increase in the number of WRKY genes in higher plant species was primarily due to tandem and segmental duplication under purifying selection. In addition, the selection pressures of different subfamilies of the WRKY gene were investigated using different strategies (classical and Bayesian maximum likelihood methods (Data monkey/PAML)). The average dN/dS for each group are less than one, indicating purifying selection. Our comparative genomic analysis provides the basis for future functional analysis, understanding the role of gene duplication in gene family expansion, and selection pressure analysis.

The 19th century proved to be one of the most complicated periods in Spanish history for the Spanish Crown, as it faced both internal conflicts—the French War of Independence—and external conflict—the independence of what were its territories in most of America. France did not remain indifferent to this and always had a clear idea of where to draw the boundaries of what “belonged” to it. Thus, amid the wave of independence movements in the Spanish colonies, the French continued to produce rich cartography to establish these boundaries and settle their power over the new nations that were arising after the period of revolutions. The cartography of Rigobert Bonne, the last cartographer of the French king and the Revolution Era, and one of its disciples, Eustache Hérisson, represent the perfect witness to the changes over the borders of the Spanish colonies during the change of the century. This study aims to analyze such cartography, examine the rich toponyms it offers, and examine the changes in the boundaries created over time between both empires. The main cartography we will rely on will be that of Bonne, one of the most important cartographers of the 18th century, and his disciple Hérisson, a geographer engineer, who lived through the onset of the conflicts and always prioritized the French perspective and the interests of their nation.

This research explores the intricate relationship between digitalization, economic development, and non-cash payments in the ASEAN-7 countries over a ten-year period from 2011 to 2020. Focusing on factors such as commercial bank branches, broad money, and inflation, the study employs panel data regression analysis to investigate their impact on automated teller machine (ATM) usage. The findings reveal that commercial bank branches significantly influence ATM usage, emphasizing the role of accessibility, services, and technological preferences. Broad money also shows a significant impact on ATM transactions, reflecting the interplay between fund availability and non-cash transactions. However, inflation does not exhibit a direct influence on ATM usage. The research underscores the importance of maintaining service quality and security in the banking sector to enhance digital financial inclusion. Future research opportunities include exploring diverse non-cash payment methods and extending studies to countries with significant global economic impacts. This research contributes valuable insights to policymakers aiming to enhance digital financial inclusion policies, ultimately fostering economic growth through the digital economy in the ASEAN-7 region.

Introduction, purpose of the study: In Central Europe, in Hungary, the state guarantees access to health care and basic health services partly through the Semmelweis Plan adopted in 2011. The Health Plan aims to optimize and transform the health system. The objectives of hospital integration, as set out in the Plan, started with the state ownership of municipal hospitals in 2012, continued with the launch of integration processes in 2012–2013 and culminated today. The transformation of a health system can have an impact on health services and thus on meeting the needs of the population. We aim to study the effectiveness of integration through access to CT diagnostic testing. Our hypothesis is that integration has resulted in increased access to modern diagnostic services. The specialty under study is computed tomography (CT) diagnostic care. Our research shows that the number of people receiving CT diagnostic care has increased significantly because of integration, which has also brought a number of positive benefits, such as reduced health inequalities, reduced travel time, costs and waiting lists. Test material and method: Our quantitative retrospective research was carried out in the hospital of Kalocsa through document analysis. The research material was comparing two time periods in the Kalocsa site of Bács-Kiskun County, Southern Hungary. The number of patients attending CT examinations by area of duty of care according to postal codes was collected: Pre-integration period 2014.01.01–2017.11.30. (Kalocsa did not have CT equipment, so patients who appeared in Kecskemét Hospital but were under the care of Kalocsa), post-integration period 2017.12.01–2019.12.31. (period after the installation of CT in Kalocsa). The target group of the study consisted of women and men together, aged 0–99 years, who appeared for a CT diagnostic examination. The study sample size was 6721 persons. Linear regression statistics were used to evaluate the results. Based on empirical experience, a SWOT analysis was carried out to further investigate the effectiveness of integration. Results: As a result of the integration, the CT scan machine purchased in the Kalocsa District Hospital has enabled an average of 129.7 patients per month to receive CT scans on site without travelling. The model used is significant, explaining 86% of the change in the number of patients served (F = 43.535; p < 0.001, adjusted R² = 0.860). The variable of integration in the model is significant, with an average increase in the number of patients served of 129.7 per month (t = 22.686; p < 0.001) following the introduction of CT due to integration. None of the month variables representing seasonal effects were found to be significant, with no seasonal effect on care. The SWOT analysis has clearly identified the strengths, weaknesses, opportunities and threats related to the integration, the main outcome of which is the acquisition of a CT diagnostic tool. Conclusions: Although we only looked at one segment of the evidence for the effectiveness of hospital integration, integration in the study area has had a positive impact on CT availability, reducing disparities in care.

Hybrid learning (HL) has become a significant part of the learning style for the higher education sector in the Sri Lankan context amidst the COVID-19 pandemic and the subsequent economic crisis. This research study aims to discover the effectiveness of hybrid learning (EHL) practices in enhancing undergraduates’ outcomes in Sri Lankan Higher Educational Institutions (HEIs) management faculties. The data for the study were gathered through an online questionnaire survey, which received 379 responses. The questionnaire contained 38 questions under four sections covering independent variables, excluding demographic questions. The results indicate that hybrid learner attitude, interaction, and benefits of hybrid learning positively impact the effectiveness of hybrid learning. The results remain consistent even after controlling for socio-demographic factors and focusing only on students employed during their higher education. The study concluded that employed students have a higher preference for the effectiveness of hybrid learning concepts, and the benefits of hybrid learning play a crucial role in enhancing the effectiveness among undergraduates. The study analyzes COVID-19’s impact on higher education, proposing hybrid learning and regulatory frameworks based on pandemic experiences while stressing the benefits of remote teaching and research.