The WRKY gene family plays a very diverse role in plant growth and development. These genes contained an evolutionarily conserved WRKY DNA binding domain, which shows functional diversity and extensive expansion of the gene family. In this study, we conducted a genome-wide comparative analysis to investigate the evolutionary aspects of the WRKY gene family across various plant species and revealed significant expansion and diversification ranging from aquatic green algae to terrestrial plants. Phylogeny reconstruction of WRKY genes was performed using the Maximum Likelihood (ML) method; the genes were grouped into seven different clades and further classified into algae, bryophytes, pteridophytes, dicotyledons, and monocotyledons subgroups. Furthermore, duplication analysis showed that the increase in the number of WRKY genes in higher plant species was primarily due to tandem and segmental duplication under purifying selection. In addition, the selection pressures of different subfamilies of the WRKY gene were investigated using different strategies (classical and Bayesian maximum likelihood methods (Data monkey/PAML)). The average dN/dS for each group are less than one, indicating purifying selection. Our comparative genomic analysis provides the basis for future functional analysis, understanding the role of gene duplication in gene family expansion, and selection pressure analysis.
The holding of soccer events has an important impact on modern urban activities, which is conducive to the economic development, social harmony, cultural integration and regional integration of cities. However, massive energy is consumed during the event preparation and infrastructure construction, resulting in an increase in the city’s carbon emissions. For the sustainable development of cities, it is important to explore the theoretical mechanism and practical effectiveness of the relationship between soccer events and urban carbon emissions, and to adopt appropriate policy management measures to control carbon emissions of soccer events. With the development of green technology, digitalization, and public transportation, the preparation and management methods of soccer events are diversified, and the possibility of carbon reduction of the event is further increased. This paper selects 17 cities in China from 2011 to 2019 and explores the complex impact of soccer events on urban carbon emissions by using green technology innovation, digitalization level and public transportation as threshold variables. The results show that: (1) Hosting soccer events increases carbon emissions with an impact coefficient of 0.021; (2) There is a negative single-threshold effect of green innovation technology, digitalization level and public transportation on the impact of soccer events on carbon emissions, with the impact coefficients of soccer events decreasing by 0.008, 0.01 and 0.06, respectively, when the threshold variable crosses the threshold. These findings will enhance the attention of city managers to the management of carbon emissions from soccer events and provide guidance for reducing carbon emissions from soccer events through green technology innovation, digital means and optimization of public transportation.
This research explores the implementation of streamlined licensing frameworks and consolidated procedures for promoting renewable energy generation worldwide. An in-depth analysis of the challenges faced by renewable energy developers and the corresponding solutions was identified through a series of industry interviews. The study aims to shed light on the key barriers encountered during project development and implementation, as well as the strategies employed to overcome these obstacles. By conducting interviews with professionals from the renewable energy sector, the research uncovers a range of common challenges, including complex permitting processes, regulatory uncertainties, grid integration issues, and financial barriers. These challenges often lead to project delays, increased costs, and limited investment opportunities, thereby hindering the growth of renewable energy generation. However, the interviews also reveal various solutions and best practices employed by industry stakeholders to address these challenges effectively. These solutions encompass the implementation of streamlined licensing procedures, such as single licenses and one-stop services, to simplify and expedite the permitting process. Additionally, the development of clear and stable regulatory frameworks, collaboration between public and private entities, and improved grid infrastructure were identified as key strategies to overcome regulatory and grid integration challenges. The research findings highlight the importance of collaborative efforts between policymakers, industry players, and other relevant stakeholders to create an enabling environment for renewable energy development. By incorporating the identified solutions and best practices, policymakers can streamline regulatory processes, foster public-private partnerships, and enhance grid infrastructure, thus catalyzing the growth of renewable energy projects.
The native peoples of the State of Mexico, especially the Mazahua community, present a high degree of marginality and food vulnerability, causing their inhabitants to be classified within the poor and extremely poor population. The objective of the research is to propose a food vulnerability index for the Mazahua community of the State of Mexico through the induction-deduction method, contrasting the existing literature with a semi-structured exploratory interview to identify the main factors that affect the native peoples. The study population was selected taking into account the number of inhabitants and poverty levels. The sources of information, in addition to documentary sources, were key informants and visits to Mazahua families that facilitated information about the different variables: natural, economic, social, cultural component, degree of adaptability and resilience for the creation and better understanding of the food vulnerability index in the communities under study.
This study meticulously explores the crucial elements precipitating corporate failures in Taiwan during the decade from 1999 to 2009. It proposes a new methodology, combining ANOVA and tuning the parameters of the classification so that its functional form describes the data best. Our analysis reveals the ten paramount factors, including Return on Capital ROA(C) before interest and depreciation, debt ratio percentage, consistent EPS across the last four seasons, Retained Earnings to Total Assets, Working Capital to Total Assets, dependency on borrowing, ratio of Current Liability to Assets, Net Value Per Share (B), the ratio of Working Capital to Equity, and the Liability-Assets Flag. This dual approach enables a more precise identification of the most instrumental variables in leading Taiwanese firms to bankruptcy based only on financial rather than including corporate governance variable. By employing a classification methodology adept at addressing class imbalance, we substantiate the significant influence these factors had on the incidence of bankruptcy among Taiwanese companies that rely solely on financial parameters. Thus, our methodology streamlines variable selection from 95 to 10 critical factors, improving bankruptcy prediction accuracy and outperforming Liang’s 2016 results.
Amid the unfolding Fourth Industrial Revolution, the integration of Logistics 4.0 with agribusiness has emerged as a pivotal nexus, harboring potential for transformational change while concurrently presenting multifaceted challenges. Through a meticulous content analysis, this systematic review delves deeply into the existing body of literature, elucidating the profound capacities of Logistics 4.0 in alleviating supply chain disruptions and underscoring its pivotal role in fostering value co-creation within agro-industrial services. The study sheds light on the transformative potential vested within nascent technologies, such as Internet of Things (IoT), Blockchain, and Artificial Intelligence (AI), and their promise in shaping the future landscape of agribusiness. However, the path forward is not without impediments; the research identifies cardinal barriers, most notably the absence of robust governmental policies and a pervasive lack of awareness, which collectively stymie the seamless incorporation of Industry 4.0 technologies within the realm of agribusiness. Significantly, this inquiry also highlights advancements in sustainable supply chain management, drawing attention to pivotal domains including digitalization, evolving labor paradigms, supply chain financing innovations, and heightened commitments to social responsibility. As we stand on the cusp of technological evolution, the study offers a forward-looking perspective, anticipating a subsequent transition towards Industry 5.0, characterized by the advent of hyper-cognitive systems, synergistic robotics, and AI-centric supply chains. In its culmination, the review presents prospective avenues for future research, emphasizing the indispensable need for relentless exploration and pragmatic solutions. This comprehensive synthesis not only sets the stage for future research endeavors but also extends invaluable insights for practitioners, policymakers, and academicians navigating the intricate labyrinthstry of Logistics 4.0 in agribusiness.
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