Cucumis sativus is an important vegetable crop in the world. Agrobacterium mediated transgenic technology is an important means to study plant gene function and variety improvement. In order to further accelerate the transgenic research and breeding process of cucumber, aiming at the Agrobacterium mediated genetic transformation method of cucumber, this paper expounds the research progress and existing problems of Agrobacterium mediated transgenic cucumber from the aspects of influencing factors of cucumber regeneration ability, genetic transformation conditions and various added substances in the process, and prospects the future of improving the efficiency of cucumber genetic transformation and the application of safety screening markers, in order to provide reference for cucumber stress resistance breeding and fruit quality improvement.

Phytomediation is an environmentally friendly green rehabilitation technology that is often incorporated with an application to improve calcium peroxide and phytohormones required for the growth of agricultural plants with the expectation to improve the effectiveness of plant rehabilitation. This study mainly consists of two parts: (1) water culture experiment and (2) pot culture experiment. In the water culture experiment, we attempt to understand the influence of the addition of calcium peroxide, phytohormones (IAA and GA₃) and a chelating agent on the growth of sunflower plants. However, in the pot culture experiment, when hormones and the chelating agent EDTA are introduced to different plant groups at the same time, if the nutrition in the water required by plants is not available, the addition of the hormone cannot negate the toxicity caused by EDTA. In terms of calcium peroxide, due to quick release of oxygen in water, this study fails to apply calcium peroxide to the water culture experiment.

When the pot culture experiment is used to examine the influence of hormones at different concentration levels on the growth of sunflowers, GA₃ 10^-8 M is reported to have the optimal effectiveness, followed by IAA 10^-8 M; IAA 10^-12 M has the lowest effectiveness. According to an accumulation analysis of heavy metals at different levels, GA₃ concentrates in leaves to transport nutrition in soil to leaves. This results in an excellent TF value of 2.329G of GA₃ than 1.845 of the control group indicating that the addition of the hormone and chelating agent to GA₃ increases the TF value and the chelating agent is beneficial to the sunflower plant. If we examine phytoattenuation ability, the one-month experiment was divided into three stages for ten days each. The concentration level of heavy metals in the soil at each stage dropped continuously while that of the control group decreased from 31.63 mg/kg to 23.96 mg/kg, GA₃ from 32.09 mg/kg to 23.04 mg/kg and EDTA from 30.65 mg/kg to 25.93 mg/kg indicating the quickest growth period of the sunflowers from the formation of the bud to blossom. During the stage, the quick upward transportation of nutrition results in quick accumulation of heavy metals; the accumulated speed of heavy metals is found higher than that of directly planted plants. This study shows an improvement in the effectiveness of the addition of hormones on plant extraction and when rehabilitation is incorporated with sunflowers with the beginning bud formation, better treatment effectiveness can be reached.

Soil and groundwater remediation act has been enacted and executed since year 2000 in Taiwan.  It has been ten good years till today where lots of remediation techniques progressively employed to improve Taiwan soil and groundwater resource quality.  Regulatory agencies, academia, remediation consulting firms, on-site professional engineers all have contribute the proud ten years in terms of soil and groundwater clean-up contribution.  However, some of technologies were un-environmental friendly even detrimental and damage to Taiwan precious soil and groundwater resources.  In Article one of the current Taiwan soil and groundwater Act, it clearly stated that soil is a precious nature resources.  Soil definitely is not a waste, shame on us most of current most commonly employed remediation are unlawful and merely aiming to save time and money consideration without any care to our land.  Dig-and-dump and soil acid washing are damaged employed in almost every single local environment agency soil clean-up project.  Lot of money, effort and time has been spent during past ten years.  Most of the spending is not improving soil quality using Green approach.

This project is carried out to assess the remediation effect on soil contaminated by molybdenum (Mo), one of heavy metals, through the use of an energy crop, sunflowers. This project explores the integration of phytohormones and chelates in the phytoremediation of soils contaminated by heavy metals, and further assesses the operational measures of remedying heavy-metal contaminated soil with sunflowers, in addition to the related environmental factors. Then the project explores phytohormones and heavy metals on the growth scenario explants (explants morphological analysis) through the experiment. The results indicate that GA₃ can increase the growth rate of the plants. The average incremental growth of the heavy-metal-added-only group is 21.0 cm; of the GA₃-added group it is 21.9 cm; of the EDDS-added group, it is 20.3 cm; of the GA₃+ EDDS-added group, it is 21.7 cm. Compared with the conventional methods of phytoremediation, these integrated measures can actually spur the growth of plants.

Soil and groundwater remediation Act was enacted in year 2000. More than ten years has already passed,  Monitoring project has been completed,pollution status has been defined,contaminated sites depollution have been launched,a great progress has been made. This paper majorly to depict the extensive farmland soil qauality monittoring which is unpredent in Taiwan and believe has never been done worldwide.

This project was initiated from February 8^th, 2002 to August 8^th, 2002. The project tasks including digitalization of cadastre, farmland listing, basic information collecting, field investigation, sampling & analysis planning, field sampling, soil sample analysis, data evaluation, suggestion of contaminated farmland control, and analysis of potential pollution sources and transfer routes.

2,251 soil samples,had been sampled from Chang-Hwa County, Yun-Lin County, Nan-Tao County, and Chia-Yi City, and been analyzed in this project. 44% of these samples concentration exceed the soil pollution control standard (Table 1), including 492 farmlands (125.65 ha registered) with total contaminated farming area of 108.38 ha in Chang-Hwa, and 6 farmlands (0.39 ha registered) with total contaminated farming area of 0.39 ha in Nan-Tao County. However, the concentration of samples from Ynu-Lin County and Chia-Yi City do not exceed the soil pollution control standard.

To coordinate with the investigation results of the relative project regarding to water and sediment quality of irrigation channels in Chang-Hwa area, the pollution sources are preliminary concluded to be the irrigation channels surrounding the farmlands in Chang-Hwa area. As to the Nan-Tao County, the abandoned brick furnace plants neighboring the farmland are suspected to be the pollution sources.

The results show that the soil of the investigation area in Chang-Hwa County is the most polluted. Base on the Geostatistics study and the distribution of the irrigation channels; the area neighboring the investigated farmland in this project is suspected being polluted. For the farmlands exceeding soil control standard, Geostatistics method is suggested to coordinate with the information of the irrigation system to clarify the contaminated area so as to be the basis of land control and remediation work. As to the farmlands, not being investigated in this project but with high pollution potential according to the Geostatistics study, detail investigations are suggested. Regarding to soil pollution remediation, it is suggested to coordinate with the effluent control and irrigation channel remediation to achieve an all-out success.