This study focused on the formulation and characterization of silver nanoparticles (AgNP) functionalized with d-limonene. The nanoparticles were functionalized by phase inversion and the synthesis of the nanoparticles was performed in situ; particle size was determined by laser diffraction, zeta potential and optical colloidal stability using Multiscan 20 for a period of 24 hours at 37 °C; the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the formulated material on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Klebsiella oxytoca ATCC 700324, Enterococcus casseliflavus ATCC 700327, Escherichia coli BLEE, carbapenem-resistant Pseudomona aeruginosa were determined. The nanoparticles showed colloidal stability at a d-limonene concentration of 3.93%, silver ions at 1.61 × 10−3%, non-ionic adjuvant at 24% and ascorbic acid at 5.88%; citric acid/citrate (1:1) 0.48M for a pH of 4.5 was used as a buffer system. The formulation was classified as a polydisperse system (PD = 0.0851), with a zeta potential of −11.6 mV and average particle size of 81.5 ± 0.9 nm. A particle migration velocity of −0.199 ± 0.006 mm∙h−1, a constant transmission profile and backscattering profile with variations of 10% were evidenced, which represents a stable formulation. The nanoparticles presented an MIC and an MBC of 28 μg∙mL−1 (5.6 × 10−2% d-limonene and 4.7 × 10−5% AgNP) against all tested bacteria.
Dust is one of the atmospheric pollutants that have adverse environmental effects and consequences. Dust fall contains particles of 100 microns or even smaller ones, which fall from the atmosphere onto the earth surface. The aim of this study is to determine the concentration of lead in dust fall samples in order to study the pollution level of this element in Zahedan, Sistan and Baluchistan Province, Iran. Therefore, sampling was carried out using 30 marble dust collectors (MDCO) for 3 months in the spring of 2015 to investigate the quantitative variation and spatial analysis of lead content in dust fall. These dust collectors were placed at 30 stations on the building roofs with a height of approximately 1.5 meters across the city. According to the results, the mean lead concentration in the spring was 90.16 mg/kg. In addition, the zoning map of lead content shows that the lowest level of lead was measured at Imam Khomeini station while the highest amount of lead appeared in Mostafa Khomeini station.
The use of saline water in agriculture is a viable alternative, considering the increased demand for fresh water. The objective of this study was to evaluate the growth and phytomass production of sugar beet under irrigation with water of different saline concentrations in a field experiment on the campus of the Federal University of Alagoas in Arapiraca. The treatments were five levels of electrical conductivity (1.0, 2.0, 3.0, 4.0 and 5.0 dS m-1). The design was in randomized blocks, with four repetitions. The maximum yield of sugar beet at 27 days after the application of saline treatments was obtained with a salinity of 3.0 dS m-1, for the variables plant height (PA), stem diameter (CD), root length (RC), aboveground dry phytomass (FSPA) and total dry phytomass (FST). At 42 days after the application of saline treatments, the variables aboveground fresh phytomass (FFPA), root fresh phytomass (FFR), total fresh phytomass (FFT), aboveground dry phytomass (FSPA) and total dry phytomass (FST) increased with increasing water salinity. Rain may have influenced the results obtained for the evaluations, performed at 42 days after the application of the saline treatments.
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