Puppetry is one of the traditional folk art forms with a long history in China.Puppetry is one of the traditional folk art forms with a long history in China. After it was transmitted to the Gaozhou Prefecture of Guangdong by the Fujian Zhangzhou Puppet Show during the Wanli period of the Ming Dynasty, it gradually took root in the local culture of Guangdong, and the Gaozhou Puppet Theatre was born as a result. Under the radiant influence of Cantonese Opera, the number one theatre in Lingnan, in the western part of Guangdong, the Gaozhou Puppet Theatre has been passed down through the generations, and has used the Cantonese Opera cantata, an element of Cantonese Opera that is the essence of the art, in its unique puppetry accent. Nowadays, when many "non-heritage" cultures are facing difficulties in inheritance and development, it is especially crucial for the puppet theatre of Gaozhou to be able to use the elements of Cantonese Opera's singing in the new era, so as to make Gaozhou Puppet Theatre a new life and make the public appreciate the art again by incorporating the elements of Cantonese Opera's singing.

The H3N2 influenza virus is spiking dramatically, which is a major concern worldwide and in India. The multifunctional hetero-trimer influenza virus RNA-dependent RNA polymerase (RdRP) is involved in the generation of viral mRNA and is crucial for viral infectivity, which is directly related to the virus’s ability to survive. The goal of the current work was to use molecular docking to determine how the RdRP protein might be affected by powerful bioactive chemicals found in Calotropis gigantia latex. By applying CB-dock 2 analysis and 2D interactions, an in-silico docking study was conducted using a GC-FID (gas chromatography with flame-ionization detection) based composition profile. Tocospiro A (15%), Amyrin (7%), and Gombasterol A were found by GC-FID to be the main phytocompounds in the latex of Calotropis gigantia. The docking result showed that ligands were effectively bound to RdRP. According to interaction studies, RdRP/ligand complexes create hydrogen bonds, van der Waals forces, pi-alkyl bonds, alkyl bonds, and pi-Sigma bonds. Therefore, it was suggested that Calotropis gigantia latex may represent a possible herbal remedy to attenuate H3N2 infections based on the above findings of the fragrance profile and docking.

Herein, we report a facile preparation of super-hydrophilic sand by coating the sand particles with cross-linked polyacrylamide (PAM) hydrogels for enhanced water absorption and controlled water release aimed at desert agriculture. To prepare the sample, 4 wt% of aqueous PAM solution is mixed with organic cross-linkers of hydroquinone (HQ) and hexamethylenetetramine (HMT) in a 1:1 weight ratio and aqueous potassium chloride (KCl) solution. A specific amount of the above solution is added to the sand, well mixed, and subsequently cured at 150 °C for 8 h. The prepared super-hydrophilic sands were characterized by Fourier-transform infrared spectroscopy (FT-IR) for chemical composition and X-ray diffraction (XRD) for successful polymer coating onto the sand. The water storage for the samples was studied by absorption kinetics at various temperature conditions, and extended water release was studied by water desorption kinetics. The water swelling ratio for the super-hydrophilic sand has reached a maximum of 900% (9 times its weight) at 80 °C within 1 h. The desorption kinetics of the samples showed that the water can be stored for up to a maximum of 3 days. Therefore, super-hydrophilic sand particles were successfully prepared by coating them with PAM hydrogels, which have great potential to be used in sustainable desert agriculture.

Health data governance is essential for optimal processing of data collection, sharing, and reuse. Although the World Health Organization (WHO) has proposed practical guidelines for managing health data during the pandemic, the Organization for Economic Cooperation and Development (OECD) found that many countries still lack the use of health data for decision-making. Therefore, this research aimed to identify and assess the challenges faced by health organization in implementing health data governance from various countries based on research articles. The challenges were assessed based on key components of health data governance from practitioner and scientist perspectives. These components include stakeholder, policy, data management, organization, data governance maturity assessment, and goals. The method used followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for collecting and reporting. Data were collected from several databases online with large repositories of academic studies, including IEEE Xplore, ScienceDirect, National Library of Medicine, ProQuest, Taylor and Francis Group, Scopus, and Wiley Online libraries. Based on the 41 papers reviewed, the results showed that policy was found to be the biggest challenge for health data governance. This was followed by data management such as quality, ownership, and access, as well as stakeholders and data governance organization. However, there were no challenges regarding maturity assessment and data governance goals, as the majority of research focused on implementation. Policy and policymaker awareness were identified as major components for the implementation of health data governance. To address challenges in data management and governance organization, creating committees focused on these components proved to be an effective solution. These results provided valuable recommendations for regulators and leaders in a healthcare organization to optimally implement health data governance.

According to the World Health Organization (WHO), breast cancer is among the most common cancers worldwide. Most of the anticancer agents have been showing a variety of side effects. Recently, bacterial proteins have been investigated as promising anticancer agents. Azurin is a bacterial cupredoxin protein secreted from Pseudomonas aeruginosa and has been reported as a potent multi-targeting anticancer agent, which makes it an appropriate candidate for drug delivery. Azurin may be delivered to cancer cells using different carriers like polymeric micro and nanoparticles. In the present study, azurin was extracted from the bacterial host and loaded into chitosan particles. Then its effect on MCF-7 cell line was investigated. Chitosan-azurin particles were made using the ion gelation method. Results showed that chitosan-azurin particles are about 200 nm, and the loading of the protein in particles did not affect its integrity. The MTT assay showed a significant reduction in cell viability in azurin and chitosan-azurin-treated cells. The toxicity level after 5 days was 63.78% and 82.53% for free azurin and chitosan-azurin-treated cells, respectively. It seems using an appropriate carrier system for anticancer proteins like azurin is a promising tool for developing low side effect anticancer agents.

The agronomic use of mushroom post-harvest substrates (SPCHs) in horticultural seedbeds could be an interesting alternative for the reuse of these wastes in line with the European circular economy strategy. This work evaluates the potential use of four treatments with different SPCHs, mushroom (-Ch), mushroom (-St), mushroom compost (-CO), and a mixture (SPCH-Ch and SPCH-St) as substrates for lettuce and chili pepper seed germination. The trial was carried out in a germination chamber using commercial compost as a control treatment. The evaluation was based on its chemical (salinity, N and C content), physical (bulk and real density, porosity and water retention) and plant effect (germination and biomass) characteristics. Of the chemical properties studied, the high salinity in SPCH-Ch and SPCH-CO was a limiting factor for the development of the horticultural species evaluated (electrical conductivity 1:2.5; p/v; ~11 dS m^-1), and low germination percentages were observed. Regarding physical properties, porosity and water retention, the SPCH-CO, SPCH-St and mixture treatments presented some values outside the optimal range established for germination substrates. In the case of SPCH-St, its high C/N ratio could be a limitation for supplying N to the crop. In relation to biomass production (aerial and root) of lettuce and chili pepper, all the treatments evaluated obtained similar values to the control treatment. The mixed treatment presented the highest biomass values, significantly higher in the lettuce crop. In general, the mixed treatment proved to be the best alternative for use in the seedbed.