According to the World Health Organization (WHO), breast cancer is among the most common cancers worldwide. Most of the anticancer agents have been showing a variety of side effects. Recently, bacterial proteins have been investigated as promising anticancer agents. Azurin is a bacterial cupredoxin protein secreted from Pseudomonas aeruginosa and has been reported as a potent multi-targeting anticancer agent, which makes it an appropriate candidate for drug delivery. Azurin may be delivered to cancer cells using different carriers like polymeric micro and nanoparticles. In the present study, azurin was extracted from the bacterial host and loaded into chitosan particles. Then its effect on MCF-7 cell line was investigated. Chitosan-azurin particles were made using the ion gelation method. Results showed that chitosan-azurin particles are about 200 nm, and the loading of the protein in particles did not affect its integrity. The MTT assay showed a significant reduction in cell viability in azurin and chitosan-azurin-treated cells. The toxicity level after 5 days was 63.78% and 82.53% for free azurin and chitosan-azurin-treated cells, respectively. It seems using an appropriate carrier system for anticancer proteins like azurin is a promising tool for developing low side effect anticancer agents.

The characteristics of agricultural products are influenced by the ecosystem, from the perspective of biotic and abiotic factors, which produce in the plant physiological responses and in turn in the fruit unique physicochemical properties, which are the basis for designations of origin and strategies to add value to the product in the current market. In the present work, ten cocoa materials (Theobroma cacao L.) were selected for their outstanding productivity (FSV41, FLE3, FEAR5, FSA12, FEC2, SCC23, SCC80, SCC55, ICS95 and CCN51), which were established in the departments of Santander (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.), Huila (931 m a.s.l.) and Huila (931 m a.s.l.). These were established in the departments of Santander (931 m a.s.l.), Huila (885 m a.s.l.) and Arauca (204 m a.s.l.), the main cocoa-producing areas in Colombia. For the evaluation of the physical characteristics of the collected materials, 21 quantitative descriptors were used to determine the physical variability of the fruit according to clone and place of collection. The data collected were analyzed by means of Pearson’s correlation matrix and principal component analysis, it was possible to identify those descriptors that contribute most to the variability among materials (ear index, diameter length ratio, seed weight and diameter, and fruit weight and length). In addition, it was possible to verify the effect of the place of harvest on the physical characteristics of the materials, high-lighting the importance of the adaptation study prior to the planting of the cocoa material, with the objective of guaranteeing a premium, productive and quality cocoa crop for the industry, which is competitive in the market.

The objective of this work was to evaluate the combined effect of bovine manure, Pseudomonas putida and Trichoderma aureoviride on the development of lettuce (Lactuca sativa). The promotion of plant growth by microorganisms may be a viable and sustainable alternative for lettuce crop management. The experimental design was entirely randomized with five treatments: T0 (witness without fertilization, P. putida and T. aureoviride), TE (cattle manure), TEB (cattle manure + P. putida), TEF (cattle manure + T. aureoviride), TEFB (cattle manure + P. putida + T. aureoviride) and ten repetitions each. The following variables were analyzed: germination velocity index (GVI), first count (FC), germination percentage (GP), leaf area index and productivity. The TEFB treatment proved to be a viable alternative for the production of lettuce, especially for small producers, since all the vegetable production in the region comes from family farming.

Objective: To evaluate the imaging features of spondyloarthritis on magnetic resonance imaging (MRI) of the sacroiliac (SI) joints in terms of topography (in thirds) and affected margin, since this aspect is rarely addressed in the literature. Methods: Cross-sectional study with MRI (1.5 T) evaluation of the SI in 16 patients with diagnosis of axial spondyloarthritis regarding the presence of acute (subchondral bone edema, enthesitis, synovitis and capsulitis) and chronic changes (erosions, subchondral bone sclerosis, bone bridging and fatty replacement), performed by two radiologists, blinded to clinical data. MRI findings were correlated with clinical data including age, disease duration, medications, HLA-B27, BASDAI, ASDAS-VHS and ASDAS-PCR, BASMI, BASFI, and mSASSS. Results: Bone edema pattern and erosions showed predominance in the upper third of SI (p = 0.050, p = 0.0014, respectively). There was a correlation between the time of disease and structural changes by affected third (p = 0.028-0.037), as well as the presence of bone bridges with BASMI (p = 0.028) and mSASSS (p = 0.014). Patients with osteitis of the lower third had higher ASDAS values (ESRV: p = 0.011 and CRP: p = 0.017). Conclusion: Chronic inflammatory changes and the pattern of bone edema predominated in the upper third of the SI, but there was also concomitant involvement of the middle or lower thirds of the joint. The localization of involvement in the upper third of the SI was insufficient to differentiate between degeneration and inflammation.

This work shows the results of the biosynthesis of silver nanoparticles using the microalga Chlorella sp, using growth media with different concentrations of glycerol, between 5%–20%, and different light and temperature conditions. The synthesis of nanoparticles was studied using supernatants and pellets from autotrophic, heterotrophic and mixotrophic cultures of the microalga. The presence of nanoparticles was verified by ultraviolet-visible spectroscopy and the samples showing the highest concentration of nanoparticles were characterized by scanning electron microscopy. The mixotrophic growth conditions favored the excretion of exopolymers that enhanced the reduction of silver and thus the formation of nanoparticles. The nanoparticles obtained presented predominantly ellipsoidal shape with dimensions of 108 nm × 156 nm and 87 nm × 123 nm for the reductions carried out with the supernatants of the mixotrophic cultures with 5% and 10% glycerol, respectively.

Map is the basic language of geography and an indispensable tool for spatial analysis. But for a long time, maps have been regarded as an objective and neutral scientific achievement. Inspired by critical geography, critical cartography/GIS came into being with the goal of clarifying the discourse embedded in cartographic practice. Power relationship challenges the untested assumption in map representation that is taken for granted. After more than 40 years of debate and running in, this research field has initially shown an outline, and critical cartography/GIS has roughly formed two research directions: the deconstruction path mainly starts from the identity of cartography subject and the process of map knowledge production, and analyzes the inseparable relationship between cartography and national governance and its internal power mechanism respectively; the construction path mainly relies on cooperative mapping and anti-mapping to realize the reproduction of map data. Domestic critical cartography/GIS research has just started, and it is necessary to continue to absorb the achievements of critical geography and carry out research in different historical periods. The deconstruction research of different types of maps also needs to strengthen the in-depth bridging between the construction path and the deconstruction path, and to be more open to the public. Impartial map application research, and actively apply the research results to social practice.