In order to seek management alternatives for anthracnose caused by the fungus Colletotrichum gloeosporioides in blackberry (Rubus glaucus Benth.), at the Tibaitatá Research Center of the Colombian Agricultural Research Corporation AGROSAVIA (formerly CORPOICA), an experiment was conducted to evaluate the effect of the application of the major elements nitrogen (N), phosphorus (P), potassium (K) and calcium (Ca) on infections of the fungus C. gloeosporioides strain-52. For this purpose, a randomized complete block design was used with an arrangement of treatments in an orthogonal central composite design. To evaluate the relationship of fertilization levels and disease severity, an artificial inoculation was made on thorny blackberry stems using 0.5 cm mycelial discs at a concentration of 9.53 × 104 conidia. Observations consisted of: disease severity (S), incubation period (IP) and rate of development (r). Data analysis was done by the cluster method on the severity variable, a Pearson correlation analysis between variables, as well as a regression to estimate the effect of nutrients applied on the severity of C. gloeosporioides strain-52. The treatments were concentrated in four groups with the ranges (in parentheses) S (15.9% and 91.8%), PI (9 and 15.3) and Tr (0.0254 and 0.0468). A positive and significant correlation was observed between S and r (P < 0.001) and a negative correlation between PI with S and r (P < 0.001). By means of regression analysis, a linear model was generated that showed a reduction in disease severity with increasing N dose and an increase with the levels of P and Ca applied.

There are numerous studies reported on the usage of the sapindus emarginatus (SE) fruit in cancer and other treatments in the past few years. In this study, crude SE fruit extract was prepared and it was further used to synthesis gold nanoparticles (Au Nps). The synthesized Au Nps were left embedded in the SE fruit extract. The Au Nps embedded in the SE fruit extract (SE-Au Nps) were characterized using UV-Visiable Spectroscopy, Centrifugal Particle Size analyzer (CPS), Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). MTT assay was carried out for both SE fruit extract and SE-Au Nps on MCF7 breast cancer cell line and thus compared. The UV-Visible Absorbance for the SE-Au Nps was obtained at 543 nm. The centrifugal particle size analysis of the Au Nps embedded in SE fruit extract showed the size of the nanoparticles to be widely varying with higher fraction of particles between the size ranges of 15 to 20 nm. The morphology of the Au Nps embedded in SE fruit extract was observed using SEM. The presence of Au Nps in SE fruit extract was confirmed using FTIR. The results of the MTT assay on MCF7 breast cancer cell line proved that the % cell viability was less for SE-Au Nps than that of the SE fruit extract alone. Thus, the antiproliferative activity of the SE fruit extract was significantly enhanced by embedding it with Au Nps and it can be effectively used in therapeutic applications after further studies.

In order to evaluate the temporal changes in tree diversity of forest vegetation in Xishuangbanna, Yunnan Province, the study collected tree diversity data from four main forest vegetation in the region through a quadrat survey including tropical rainforest (TRF), tropical coniferous forest (COF), tropical lower mountain evergreen broad-leaved forest (TEBF), tropical seasonal moist forest (TSMF). We extracted the distribution of four forest vegetation in the region in four periods of 1992, 2000, 2009, and 2016 in combination with remote sensing images, using simp son Shannon Wiener and scaling species diversity indexes compare to the differences of tree evenness of four forest vegetation and use the scaling ecological diversity index and grey correlation evaluation model to evaluate the temporal changes of forest tree diversity in the region in four periods. The results show that: (1) The proportion of forest area has a trend of decreasing first and then increasing, which is shown by the reduction from 65.5% in 1992 to 53.42% in 2000, to 52.49% in 2009, and then to 54.73% in 2016. However, the tropical rainforest shows a continuous decreasing trend. (2) There are obvious differences in the contributions of the four kinds of forest vegetation to tree diversity. The order of evenness is tropical rainforest > tropical mountain (low mountain) evergreen broad-leaved forest > warm coniferous forest > tropical seasonal humid forest, and the order of richness is tropical rainforest > tropical mountain (low mountain) evergreen broad-leaved forest > tropical seasonal humid forest > warm coniferous forest, The order of contribution to tree diversity in tropical rainforest > tropical mountain (low mountain) evergreen broad-leaved forest > tropical seasonal humid forest > warm tropical coniferous forest. (3) The tree diversity of tropical rainforests and tropical seasonal humid forests showed a continuous decreasing trend. The tree diversity of forest vegetation in Xishuangbanna in four periods was 1992 > 2009 > 2016 > 2000. The above results show that economic activities are an important factor affecting the biodivesity of Xishuangbanna, and the protection of tropical rainforest is of great significance to maintain the biodiversity of the region.