The fresh dried pollen of grape and seedless grape varieties were used as the research material. Each cultivar was stored at room temperature, 5 C, 0 C, -18 C and -40 C respectively. Sucrose 200g / L + boric acid 50mg / L + agar 8g / L as the nutrient substrate, and the comparative study on the germination and culture of the nuclear breed and the seedless cultivar. The results showed that the pollen viability decreased with the increase of storage time at different temperatures. The pollen viability decreased at -18 C and -40 , and the pollen viability decreased at the same temperature The There was a significant difference in pollen viability between different cultivars at the beginning of storage, and the pollen viability of the kernel breed was higher than that of the seedless grape.

Background: Through the development of robust techniques and their comprehensive validation, cardiac magnetic resonance imaging (CMR) has developed a wide range of indications in its almost 25 years of clinical use. The recording of cardiac volumes and systolic ventricular function as well as the characterization of focal myocardial scars are now part of standard CMR imaging. Recently, the introduction of accelerated image acquisition technologies, the new imaging methods of myocardial T1 and T2 mapping and 4-D flow measurements, and the new post-processing technique of myocardial feature tracking have gained relevance. Method: This overview is based on a comprehensive literature search in the PubMed database on new CMR techniques and their clinical application. Results and conclusion: This article provides an overview of the latest technical developments in the field of CMR and their possible applications based on the most important clinical questions.