A comparative study on the pollen viability and storage  conditions of the nuclear and non - nuclear varieties

Shasha Tang; Yuling Huang; Xiao Fu

doi:10.24294/th.v1i1.241

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A comparative study on the pollen viability and storage conditions of the nuclear and non - nuclear varieties

Shasha Tang

Yuling Huang

Xiao Fu

Trends in Horticulture 2018, 1(1), 241; https://doi.org/10.24294/th.v1i1.241

Submitted：01 Feb 2018

Accepted：07 Feb 2018

Published：07 Feb 2018

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Abstract

The fresh dried pollen of grape and seedless grape varieties were used as the research material. Each cultivar was stored at room temperature, 5 C, 0 C, -18 C and -40 C respectively. Sucrose 200g / L + boric acid 50mg / L + agar 8g / L as the nutrient substrate, and the comparative study on the germination and culture of the nuclear breed and the seedless cultivar. The results showed that the pollen viability decreased with the increase of storage time at diﬀerent temperatures. The pollen viability decreased at -18 C and -40 , and the pollen viability decreased at the same temperature The There was a significant diﬀerence in pollen viability between diﬀerent cultivars at the beginning of storage, and the pollen viability of the kernel breed was higher than that of the seedless grape.

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