The fresh dried pollen of grape and seedless grape varieties were used as the research material. Each cultivar was stored at room temperature, 5 C, 0 C, -18 C and -40 C respectively. Sucrose 200g / L + boric acid 50mg / L + agar 8g / L as the nutrient substrate, and the comparative study on the germination and culture of the nuclear breed and the seedless cultivar. The results showed that the pollen viability decreased with the increase of storage time at different temperatures. The pollen viability decreased at -18 C and -40 , and the pollen viability decreased at the same temperature The There was a significant difference in pollen viability between different cultivars at the beginning of storage, and the pollen viability of the kernel breed was higher than that of the seedless grape.

Lianghuai Mining Area is one of the 13 large coal bases in China. It is an important coal and coal production base in China. Mine water inrush accidents occur frequently, resulting in economic and human resource losses, reflecting the importance of the study of hydrogeology in mining areas. In this paper, the hydrogeological conditions of Bozhou and Huainan Panxie mine are analyzed, and the similarities and differences between the hydrogeological conditions of the two mines are summarized. The shallow pore water group in the Bozhou area is composed of the Quaternary system of the Quaternary system (Q4d) and the upper part of the upper part of the Mao Tong group (Q3m). The lithology of the aquifer is silt, silt and fine sand. The shallow pore water group of the Panxian Pancho Formation in Huainan is composed of the Upper Pleistocene of the Quaternary system and the Holocene strata. The lithology is mainly composed of fine sand. The main sources of shallow pore water supply in the two areas are precipitation infiltration, mainly for evaporation, lateral runoff, artificial mining and deep flow and discharge to the river.