Cysteine is one of the body’s essential amino acids to build proteins. For the early diagnosis of a number of diseases and biological issues, L-cysteine (L-Cys) is essential. Our study presents an electrochemical sensor that detects L-cysteine by immobilizing the horseradish peroxidase (HRP) enzyme on a reduced graphene oxide (GCE) modified glassy carbon electrode. The morphologies and chemical compositions of synthesized materials were examined using Fourier transform infrared spectroscopy (FTIR) and field-emission scanning electron microscopy (FESEM). The modified electrode’s electrochemical behavior was investigated using cyclic voltammetry (CV). Cyclic voltammetry demonstrated HRP/rGO/GCE has better electrocatalytic activity than bare GCE in the oxidation of L-cysteine oxidation in a solution of acetate buffer. The electrochemical sensor had a broad linear range of 0 µM to 1 mM, a 0.32 µM detection limit, and a sensitivity of 6.08 μA μM⁻¹ cm⁻². The developed sensor was successfully used for the L-cysteine detection in a real blood sample with good results.

The intermittent flow cold storage heat exchanger is one of the most important components of the pulse tube expansion refrigerator based on the reverse Brayton cycle. In the experimental system, the volume and heat transfer of the helical tube play a decisive role in the stable operation of the whole experimental system. However, there are few studies on heat transfer in a helical tube under helium working medium and intermittent flow conditions. In this paper, a process and method for calculating the volume of a helical tube are proposed based on the gas vessel dynamics model. Subsequently, a three-dimensional simulation model of the helical tube was established to analyze the heat transfer process of cryogenic helium within the tube. The simulations revealed that the temperature of helium in the tube decreases to the wall temperature and does not change when the helical angle exceeds 720°. Moreover, within the mass flow rate range of 1.6 g/s to 3.2 g/s, an increase in the mass flow rate was found to enhance the heat transfer performance of the helical tube. This study provides a reference for the selection and application of a helical tube under intermittent flow conditions and also contributes to the experimental research of inter-wall heat exchanger and pulse tube expansion refrigerators.

In an effort to bridge the gap of economic and social inequality among the community, rural areas in Indonesia are encouraged to be self-sufficient in generating income. This makes the central government create various policies so that the regional government maximizes the management of its potential as an economic resource for the well-being of its people. One of the ways to manage this potential is to encourage rural areas to create tourism products that can be sold to the public. The Indonesian governments openly use the tourism sector as a tool for the development in many rural areas. Next, efforts to achieve successful development of the district will be closely related to the strategic planning and long-term cooperation of each local government with stakeholders in its implementation. These two points are the basic elements of the new regionalism theory. This theory states that the role of local governments is very important in initiating and making policies for new economic activities for a significant improvement in the quality of their population. Therefore, this study tries to explore how the new theory of regionalism can include rural development from a tourism perspective as a way to stimulate the fading economy in rural area of Indonesia. The study found that the new theory of regionalism needs support from various aspects such as social-cultural, community participation, the three pillars of sustainable development namely economic, social, and environmental as well as basic aspects to shape sustainable rural development through tourism.

Soil salinity is a major abiotic stress that drastically hinders plant growth and development, resulting in lower crop yields and productivity. As one of the most consumed vegetables worldwide, tomato (Solanum lycropersicum L.) plays a key role in the human diet. The current study aimed to explore the differential tolerance level of two tomato varieties (Rio Grande and Agata) to salt stress. To this end, various growth, physiological and biochemical attributes were assessed after two weeks of 100 mM NaCl treatment. Obtained findings indicated that, although the effects of salt stress included noticeable reductions in shoots’ and roots’ dry weights and relative growth rate as well as total leaf area, for the both cultivars, Rio Grande performed better compared to Agata variety. Furthermore, despite the exposure to salt stress, Rio Grande was able to maintain an adequate tissue hydration and a high leaf mass per area (LMA) through the accumulation of proline. However, relative water content, LMA and proline content were noticeably decreased for Agata cultivar. Likewise, total leaf chlorophyll, soluble proteins and total carbohydrates were significantly decreased; whereas, malondialdehyde was significantly accumulated in response to salt stress for the both cultivars. Moreover, such negative effects were remarkably more pronounced for Agata relative to Rio Grande cultivar. Overall, the current study provided evidence that, at the early growth stage, Rio Grande is more tolerant to salt stress than Agata variety. Therefore, Rio Grande variety may constitute a good candidate for inclusion in tomato breeding programs for salt-tolerance and is highly recommended for tomato growers, particularly in salt-affected fields.

This article presents a methodology to perform quality analysis on the cadastral map, based on the tools provided by open (public or free) license geographic information systems (GIS). The errors presented in the cadastral map have a direct impact on the information systems, which can lead to erroneous decisions and to an increase in the costs of maintenance and updating of spatial data. The methodology developed was used and tested by Costa Rica’s Cadastre and Registry Regularization Program; as a product of this program, a continuous cadastral map has been created for Costa Rica, on which cadastral and registry transactions will be processed within the National Registry of Costa Rica. The methodology allows detecting, locating and classifying errors in the cadastral map for easily correcting, so that this map correctly represents the reality of the properties that conform it.