In this investigation the effect of collection seasons of explants (winter, spring and summer), type of explants (leaf disc and intermodal segments) and length of explants (0.5, 1.0 and 1.5 cm) for callusing in low-chill peach were standardized. The maximum callus induction (97.78%) in the low-chill peach was obtained from the intermodal segments of 0.5 cm in length used as an explant collected during spring season. The structural changes on the surface of the callus (5–7 weeks old yellowish green compact callus) during the progress of somatic embryogenesis of low-chill peach from the both intermodal segment as well as leaf disc derived callus were also examined with the use of scanning electron microscope (SEM). The SEM studies indicated that callus derived from internodal segment explant had the highest frequency of somatic embryos than callus from leaf discs. The SEM investigation, also demonstrated the sequential events/steps leading to low-chill peach somatic embryogenesis which was originating from somatic embryo mother cells through one unicellular pathway. Two types of calli were morphologically distinguished in both leaf disc and intermodal segment generated callus and these were the compact, well organized yellowish green embryogenic callus, containing large number of small, rich cytoplasmic, starch containing meristematic cells and soft and unorganized non-embryogenic callus containing sparsely cytoplasmic, vacuolated, and large cells devoid of metabolic reserves. The present SEM studies clearly demonstrated that somatic cells from peach explants generated callus could develop into fully differentiated somatic embryos through the characteristic embryological patterns of differentiation.

The WRKY gene family plays a very diverse role in plant growth and development. These genes contained an evolutionarily conserved WRKY DNA binding domain, which shows functional diversity and extensive expansion of the gene family. In this study, we conducted a genome-wide comparative analysis to investigate the evolutionary aspects of the WRKY gene family across various plant species and revealed significant expansion and diversification ranging from aquatic green algae to terrestrial plants. Phylogeny reconstruction of WRKY genes was performed using the Maximum Likelihood (ML) method; the genes were grouped into seven different clades and further classified into algae, bryophytes, pteridophytes, dicotyledons, and monocotyledons subgroups. Furthermore, duplication analysis showed that the increase in the number of WRKY genes in higher plant species was primarily due to tandem and segmental duplication under purifying selection. In addition, the selection pressures of different subfamilies of the WRKY gene were investigated using different strategies (classical and Bayesian maximum likelihood methods (Data monkey/PAML)). The average dN/dS for each group are less than one, indicating purifying selection. Our comparative genomic analysis provides the basis for future functional analysis, understanding the role of gene duplication in gene family expansion, and selection pressure analysis.

Governments intervene in the housing market via implementing various monetary, fiscal, foreign exchange and credit policies. By this, the housing market undergoes cycles of boom and bust as well as significant swings in value added and housing prices. Therefore, the main goal of this research is to consider the effect of the government’s change on the monetary and financial policy’s impact on the business cycles of the housing sector during the period of 1978–2020. On the other hand, we estimate the impact of monetary and fiscal policies on housing business cycles concerning government’s change. To calculate housing business cycles (boom and busts), the housing value added were initially de-trended using the Hodrick–Prescott filter. This paper takes a novel use of the threshold regression model with government’s change as threshold variable. According to the study’s findings, there are three threshold effects (two threshold levels or three regimes) of monetary and fiscal policy on housing business cycles. For instance, the money supply coefficient in the first regime was −1.68, indicating that the effect of monetary policy in this regime is countercyclical. in the second and third regimes, it was 0.19 and 0.03, respectively; indicating its alignment with the housing business cycle. Regarding the estimated models, we may derive several interesting conclusions. In first regime, the money supply is countercyclical and government expenditure is pro-cyclical. This means that monetary policy exacerbates recession and fiscal policy weakens it. in the second and third regimes, the money supply is pro-cyclical and government expenditure is countercyclical. As a result, while formulating their monetary policies, governments should give the housing sector more consideration. Additionally, when putting this policy into practice, the housing sector has to be carefully examined.

In the domains of geological study, natural resource exploitation, geological hazards, sustainable development, and environmental management, lithological mapping holds significant importance. Conventional approaches to lithological mapping sometimes entail considerable effort and difficulties, especially in geographically isolated or inaccessible regions. Incorporating geological surveys and satellite data is a powerful approach that can be effectively employed for lithological mapping. During this process, contemporary RS-enhancing methodologies demonstrate a remarkable proficiency in identifying complex patterns and attributes within the data, hence facilitating the classification of diverse lithological entities. The primary objective of this study is to ascertain the lithological units present in the western section of the Sohag region. This objective will be achieved by integrating Landsat ETM⁺ satellite imagery and field observations. To achieve our objectives, we employed many methodologies, including the true and false color composition (FCC&TCC), the minimal noise fraction (MNF), principal component analysis (PCA), decoration stretch (DS), and independent component analysis (ICA). Our findings from the field investigation and the data presented offer compelling evidence that the distinct lithological units can be effectively distinguished. A recently introduced geology map has been incorporated within the research area. The sequence of formations depicted in this map is as follows: Thebes, Drunka, Katkut, Abu Retag, Issawia, Armant, Qena, Abbassia, and Dandara. Implementing this integrated technique enhances our comprehension of geological units and their impacts on urban development in the area. Based on the new geologic map of the study area, geologists can improve urban development in the regions by detecting building materials “aggregates”. This underscores the significance and potential of our research in the context of urban development.

The experiments were carried out to validate an analytical method and to examine the impact of various decontaminating solutions on the removal of acephate residues from okra. Acephate analysis was performed using HPLC-UV, and sample extraction was done using the QuEChERS method. Method validation encompassed assessing specificity, linearity, precision, accuracy, as well as limits of detection (LOD) and quantification (LOQ). The method exhibited excellent linearity with R² values ≥ 0.99. LOD and LOQ were determined at 0.5 µg mL⁻¹ and 2 µg mL⁻¹, respectively. The results indicated average recoveries ranging from 80.2% to 83.3% with a % RSD below 5%. The decontamination procedures include rinsing with running tap water, soaking in lukewarm water, 2% CH₃COOH, 1% NaCl, 5% NaHCO₃, 0.01% KMnO₄, and in commercially available decontamination products such as nimwash, veggie clean, and arka herbiwash for a duration 10 minutes. Among all the treatments, soaking in nimwash solution showed remarkable effectiveness (96.75% removal), followed by veggie clean (94.97% removal) and arka herbiwash (95.80% removal). Washing okra samples in running tap water was found to be the least effective compared to other treatments.

Focusing on Shanghai Port, this in-depth study explores how government support can make port organizations more competitive. This study shall implement qualitative analysis based on in-depth interviews with key industry and government leaders to break down the complicated actions taken by the government and how they have changed the operational and strategic skills of the port industry. Seven factors were found in our study to be the most crucial support factors: Financial, regulatory, infrastructure growth, talent, market, policy, and organizational support. In their ways, each of these groups undermines the ability of port businesses to compete. For instance, finance can make ports more competitive in aspects such as tax cuts, lower interest rates, innovation and R&D funds, financing programs, venture capital funds, and putting up R&D sites. Supporting regulations makes sure that there is fair competition and smooth operations. This is done by protecting intellectual property, keeping the market going smoothly, improving the business environment, and monitoring market regulations. Building new infrastructure, such as innovation and updated buildings, enables the smooth running of the port businesses and minimizes wastage of time; thus, more time is spent on production. Supporting talent, the market, and policy all work together to make the human capital, international cooperation, and strategic regulatory framework that a company needs to stay ahead in the long run. It is clear from organizational support how important collaborative networks are for making ports more competitive. These networks, for instance, can be of assistance in helping schools and businesses work together, create new technologies, and find ways for companies and colleges to study together. This study examines these support systems to determine where the government should step in and how the systems can be made better to make ports more competitive. In terms of practical contribution, this in-depth study helps policymakers and port workers plan for the future. This study shows a fair way for the government to support the port business, which changes with its needs and stays competitive in the world of trade.